eCite Digital Repository

The floral transcriptome of Eucalyptus grandis

Citation

Vining, KJ and Romanel, E and Jones, RC and Klocko, A and Alves-Ferreira, M and Hefer, CA and Amarasinghe, V and Dharmawardhana, P and Naithani, S and Ranik, M and Wesley-Smith, J and Solomon, L and Jaiswal, P and Myburg, AA and Strauss, SH, The floral transcriptome of Eucalyptus grandis, New Phytologist, 206, (4) pp. 1406-1422. ISSN 0028-646X (2015) [Refereed Article]

Copyright Statement

Copyright 2014 New Phytologist

DOI: doi:10.1111/nph.13077

Abstract

  • As a step toward functional annotation of genes required for floral initiation and development within the Eucalyptus genome, we used short read sequencing to analyze transcriptomes of floral buds from early and late developmental stages, and compared these with transcriptomes of diverse vegetative tissues, including leaves, roots, and stems.
  • A subset of 4807 genes (13% of protein-coding genes) were differentially expressed between floral buds of either stage and vegetative tissues. A similar proportion of genes were differentially expressed among all tissues. A total of 479 genes were differentially expressed between early and late stages of floral development. Gene function enrichment identified 158 gene ontology classes that were overrepresented in floral tissues, including ‘pollen development’ and ‘aromatic compound biosynthetic process’. At least 40 floral-dominant genes lacked functional annotations and thus may be novel floral transcripts.
  • We analyzed several genes and gene families in depth, including 49 putative biomarkers of floral development, the MADS-box transcription factors, ‘S-domain’-receptor-like kinases, and selected gene family members with phosphatidylethanolamine-binding protein domains. Expanded MADS-box gene subfamilies in Eucalyptus grandis included SUPPRESSOR OF OVEREXPRESSION OF CO 1 (SOC1), SEPALLATA (SEP) and SHORT VEGETATIVE PHASE (SVP) Arabidopsis thaliana homologs.
  • These data provide a rich resource for functional and evolutionary analysis of genes controlling eucalypt floral development, and new tools for breeding and biotechnology.
  • Item Details

    Item Type:Refereed Article
    Keywords:Eucalyptus, floral transcriptome, flowering, MADS-box
    Research Division:Biological Sciences
    Research Group:Genetics
    Research Field:Gene Expression (incl. Microarray and other genome-wide approaches)
    Objective Division:Plant Production and Plant Primary Products
    Objective Group:Forestry
    Objective Field:Hardwood Plantations
    Author:Jones, RC (Dr Rebecca Jones)
    ID Code:97736
    Year Published:2015 (online first 2014)
    Web of Science® Times Cited:7
    Deposited By:Plant Science
    Deposited On:2015-01-12
    Last Modified:2016-02-12
    Downloads:0

    Repository Staff Only: item control page