The purification and fractionation of phenolic compounds from crude plant extracts using a food-grade acrylic adsorbent were studied at pilot-plant scale. A diluted apple juice concentrate served as a model phenolic solution for column adsorption and desorption trials. Phenolic concentrations were evaluated photometrically using the Folin−Ciocalteu assay and by HPLC-DAD. Recovery rates were significantly affected by increasing phenolic concentrations of the feed solutions applied to the column. In contrast, the flow rate during column loading hardly influenced adsorption efficiency, whereas the temperature and pH value were shown to be crucial parameters determining both total phenolic recovery rates and the adsorption behavior of individual polyphenols. As expected, the eluent composition had the greatest impact on the desorption characteristics of both total and individual phenolic compounds. HPLC analyses revealed significantly different elution profiles of individual polyphenols depending on lipophilicity. This technique allows fractionation of crude plant phenolic extracts, thus providing the opportunity to design the functional properties of the resulting phenolic fractions selectively, and the present study delivers valuable information with regard to the adjustment of individual process parameters.

Item Type:	Refereed Article
Keywords:	apple polyphenols, adsorption, desorption, adsorbent resin, recovery, fractionation
Research Division:	Agricultural, Veterinary and Food Sciences
Research Group:	Crop and pasture production
Research Field:	Crop and pasture biochemistry and physiology
Objective Division:	Plant Production and Plant Primary Products
Objective Group:	Horticultural crops
Objective Field:	Pome fruit, pip fruit
UTAS Author:	Stanley, RA (Professor Roger Stanley)
ID Code:	97682
Year Published:	2010
Web of Science® Times Cited:	54
Deposited By:	Tasmanian Institute of Agriculture
Deposited On:	2015-01-08
Last Modified:	2015-02-10
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