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Pilot-scale resin adsorption as a means to recover and fractionate apple polyphenols
journal contribution
posted on 2023-05-18, 06:12 authored by Kammerer, DR, Carle, R, Roger StanleyRoger Stanley, Saleh, ZSThe purification and fractionation of phenolic compounds from crude plant extracts using a food-grade acrylic adsorbent were studied at pilot-plant scale. A diluted apple juice concentrate served as a model phenolic solution for column adsorption and desorption trials. Phenolic concentrations were evaluated photometrically using the Folin−Ciocalteu assay and by HPLC-DAD. Recovery rates were significantly affected by increasing phenolic concentrations of the feed solutions applied to the column. In contrast, the flow rate during column loading hardly influenced adsorption efficiency, whereas the temperature and pH value were shown to be crucial parameters determining both total phenolic recovery rates and the adsorption behavior of individual polyphenols. As expected, the eluent composition had the greatest impact on the desorption characteristics of both total and individual phenolic compounds. HPLC analyses revealed significantly different elution profiles of individual polyphenols depending on lipophilicity. This technique allows fractionation of crude plant phenolic extracts, thus providing the opportunity to design the functional properties of the resulting phenolic fractions selectively, and the present study delivers valuable information with regard to the adjustment of individual process parameters.
History
Publication title
Journal of Agricultural and Food ChemistryVolume
58Issue
11Pagination
6787-6796ISSN
1520-5118Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
American Chemical SocietyPlace of publication
United States of AmericaRights statement
Copyright 2010 American Chemical SocietyRepository Status
- Restricted