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Effects of single and repeated infections with Neoparamoeba perurans on antibody levels and immune gene expression in Atlantic salmon (Salmo salar)

Citation

Valdenegro-Vega, VA and Polinski, M and Bridle, A and Crosbie, P and Leef, M and Nowak, BF, Effects of single and repeated infections with Neoparamoeba perurans on antibody levels and immune gene expression in Atlantic salmon (Salmo salar), Fish and Shellfish Immunology, 42, (2) pp. 522-529. ISSN 1050-4648 (2015) [Refereed Article]

Copyright Statement

Copyright 2014 Elsevier Ltd.

DOI: doi:10.1016/j.fsi.2014.11.031

Abstract

Amoebic gill disease (AGD) is the main health problem for the salmon industry in Tasmania, Australia and is now reported in most salmon producing countries. Antibody and gene expression responses to the pathogen, Neoparamoeba perurans, have been studied independently following primary exposure; however, the effects of sequential reinfection, which can often occur during net-pen culture of salmon, remain unclear. The association between the transcription of immunoglobulin (Ig) and their systemic and mucosal antibody levels in regards to AGD is unknown. Herein, we assessed the antibody responses as well as Ig transcription in the gills of Atlantic salmon infected only once and also sequentially with N.áperurans. After four successive AGD challenges, no significant differences in plasma or skin mucus levels of IgM were observed between AGD-na´ve and challenged fish. However, IgM gene expression in gill lesions of AGD-affected fish increased up to 31ád after infection, while no changes in IgT, TCR and CD8 transcription were observed. Changes at IgM transcription level did not match the lack of antibody response in mucus, which is possibly explained by weak correlations existing between protein and mRNA abundances in cells and tissues. In the second experiment, which investigated Ig responses to AGD at the transcriptional as well as antibody production level in salmon after a single infection, the levels of serum or skin mucus IgM antibody were not affected and no changes in the IgM or IgT transcription were induced.

Item Details

Item Type:Refereed Article
Keywords:AGD, gene expression, IgM, IgT, antibody levels
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Fin Fish (excl. Tuna)
Author:Valdenegro-Vega, VA (Miss Victoria Valdenegro)
Author:Polinski, M (Mr Mark Polinski)
Author:Bridle, A (Dr Andrew Bridle)
Author:Crosbie, P (Dr Philip Crosbie)
Author:Leef, M (Dr Melanie Leef)
Author:Nowak, BF (Professor Barbara Nowak)
ID Code:97603
Year Published:2015
Web of Science® Times Cited:5
Deposited By:IMAS Research and Education Centre
Deposited On:2015-01-02
Last Modified:2016-04-13
Downloads:0

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