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Overdominance effect of the bovine ghrelin receptor (GHSR1a)-DelR242 locus on growth in Japanese Shorthorn weaner bulls: heterozygote advantage in bull selection and molecular mechanisms

Citation

Komatsu, M and Sato, Y and Negami, T and Terada, T and Sasaki, O and Yasuda, J and Arakawa, A and Yoshida, C and Takahashi, H and Malau-Aduli, AEO and Suzuki, K and Shimizu, K, Overdominance effect of the bovine ghrelin receptor (GHSR1a)-DelR242 locus on growth in Japanese Shorthorn weaner bulls: heterozygote advantage in bull selection and molecular mechanisms, G3: Genes, Genomes, Genetics, 5, (2) pp. 271-279. ISSN 2160-1836 (2015) [Refereed Article]


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Copyright 2014 Komatsu et al. Licenced under Creative Commons Attribution 3.0 Unported (CC BY 3.0) http://creativecommons.org/licenses/by/3.0/

DOI: doi:10.1534/g3.114.016105

Abstract

Ghrelin and the ghrelin receptor (GHSR1a) are involved in growth hormone secretion, food intake, and several other important functions. Ghrelin acts on GHSR1a and induces signal transduction via the Gαq subunit. In our previous study, we identified the DelR242 (3R) allele, a truncated 3-arginine residue (3R) [major type: 4 arginine residues (4R)] of the third intracellular loop of GHSR1a, with a high frequency in Japanese Shorthorn bulls (0.43) but with a low frequency in other cattle breeds (0.00-0.09). To further investigate the reasons for the higher frequency of the 3R allele, we performed several experiments. In this study, we found a significant sex difference in the frequency of the 3R allele. Statistical analysis revealed a significant overdominance effect of the DelR242 locus on growth in Japanese Shorthorn weaner bulls. However, additive/dominance/overdominance effects of the 3R allele on carcass traits in adult steers and dams were not significant. The mode of the overdominance effect was estimated to be solely controlled by the single DelR242 locus without any other linked loci using linkage disequilibrium analysis in GHSR1a. These results indicated that 4R/3R heterozygotes had a selective advantage in weaner bulls because of their higher average daily gain than homozygotes. We discussed possible molecular mechanisms involved in the overdominance effect of the DelR242 locus on these traits in weaner bulls using a structural model of the complex consisting of a GHSR1a dimer and Gαq.

Item Details

Item Type:Refereed Article
Keywords:overdominance, growth, ghrelin receptor (GHSR1a), DelR242, cattle
Research Division:Agricultural and Veterinary Sciences
Research Group:Animal Production
Research Field:Animal Breeding
Objective Division:Animal Production and Animal Primary Products
Objective Group:Livestock Raising
Objective Field:Beef Cattle
Author:Malau-Aduli, AEO (Associate Professor Aduli Malau-Aduli)
ID Code:97535
Year Published:2015
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2014-12-22
Last Modified:2016-05-06
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