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Acute diesel exhaust particle exposure increases viral titre associated with influenza but does not exacerbate inflammation or deficits in lung function

conference contribution
posted on 2023-05-24, 13:24 authored by Foong, RE, Boylen, CE, Larcombe, AN, Graeme ZoskyGraeme Zosky

Introduction: Diesel exhaust particles (DEP) make up the bulk of particulate matter in urban areas. High ambient levels of particulate matter are associated with increased hospitalization due to respiratory disease. We aimed to determine if exposure to DEP exacerbates responses to acute viral infection.

Methods: Adult female BALB/c mice were inoculated with 100 μg DEP or control 3.75 days after infection with 104.5 plaque forming units (pfu) of infl uenza A/Mem71 (or control). Six hours after DEP inoculation, lung volume (TGV) and lung mechanics were measured by plethysmography and the forced oscillation technique, respectively. Bronchoalveolar lavage fl uid was collected to assess cellular infl ammation and cytokine levels.

Results: Viral titre was signifi cantly higher in infl uenza-infected mice exposed to DEP compared to those exposed to infl uenza alone (p = 0.04). Both DEP (p = 0.03) and infl uenza infection (p < 0.001) alone signifi cantly increased cellular infl ammation; however, there was no difference between mice exposed to both DEP and infl uenza compared to those exposed to infl uenza alone (p = 0.42). A similar pattern was found in levels of cytokines in the bronchoalveolar lavage (TNF-α, MCP-1, IL-6, IFN-γ). Specific airway resistance, specific tissue damping, specifi c tissue elastance and hysteresivity were significantly increased in influenza infected mice (p < 0.001 in all cases). None of these parameters were influenced by DEP exposure alone (p > 0.33 in all cases) and there was no additive effect of DEP on lung function (p > 0.22 in all cases) in influenza-infected mice.

Conclusions

: DEP increases viral titre but is not sufficient to physiologically exacerbate pre-existing respiratory disease caused by infl uenza infection in mice.

History

Publication title

Respirology

Volume

16 (Suppl.1)

Editors

P Bardin

Pagination

43

ISSN

1323-7799

Department/School

Tasmanian School of Medicine

Publisher

Wiley-Blackwell Publishing Asia

Place of publication

Australia

Event title

TSANZ ASM 2011

Event Venue

Perth, Australia

Date of Event (Start Date)

2011-04-02

Date of Event (End Date)

2011-04-06

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

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