Knopfel, T and Lin, MZ and Levskaya, A and Lin, JY and Boyden, ES, Toward the Second Generation of Optogenetic Tools, Journal of Neuroscience, 30, (45) pp. 14998 -15004. ISSN 0270-6474 (2010) [Substantial Review]
This mini-symposium aimsto provide an integrated perspective on recent developments in optogenetics. Research inthis emerging field combines optical methods with targeted expression of genetically encoded, protein-based probes to achieve experimental manipulation and measurement of neural systems with superior temporal and spatial resolution. The essential components of the optogenetic toolbox consist of two kinds of molecular devices: actuators and reporters, which respectively enable light-mediated control or monitoring of molecular processes. The first generation of genetically encoded calcium reporters, fluorescent proteins, and neural activators has already had a great impact on neuroscience. Now, a second generation of voltage reporters, neural silencers, and functionally extended fluorescent proteins hold great promise for continuing this revolution. In this review, we will evaluate and highlight the limitations of presently available optogenic tools and discuss where these technologies and their applications are headed in the future.