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Toward the Second Generation of Optogenetic Tools

journal contribution
posted on 2023-05-22, 23:44 authored by Knopfel, T, Lin, MZ, Levskaya, A, Lin, JY, Boyden, ES
This mini-symposium aimsto provide an integrated perspective on recent developments in optogenetics. Research inthis emerging field combines optical methods with targeted expression of genetically encoded, protein-based probes to achieve experimental manipulation and measurement of neural systems with superior temporal and spatial resolution. The essential components of the optogenetic toolbox consist of two kinds of molecular devices: actuators and reporters, which respectively enable light-mediated control or monitoring of molecular processes. The first generation of genetically encoded calcium reporters, fluorescent proteins, and neural activators has already had a great impact on neuroscience. Now, a second generation of voltage reporters, neural silencers, and functionally extended fluorescent proteins hold great promise for continuing this revolution. In this review, we will evaluate and highlight the limitations of presently available optogenic tools and discuss where these technologies and their applications are headed in the future.

History

Publication title

Journal of Neuroscience

Volume

30

Issue

45

Pagination

14998 -15004

ISSN

0270-6474

Publisher

Soc Neuroscience

Place of publication

11 Dupont Circle, Nw, Ste 500, Washington, USA, Dc, 20036

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the biological sciences

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