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Toward the Second Generation of Optogenetic Tools


Knopfel, T and Lin, MZ and Levskaya, A and Lin, JY and Boyden, ES, Toward the Second Generation of Optogenetic Tools, Journal of Neuroscience, 30, (45) pp. 14998 -15004. ISSN 0270-6474 (2010) [Substantial Review]

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DOI: doi:10.1523/JNEUROSCI.4190-10.2010


This mini-symposium aimsto provide an integrated perspective on recent developments in optogenetics. Research inthis emerging field combines optical methods with targeted expression of genetically encoded, protein-based probes to achieve experimental manipulation and measurement of neural systems with superior temporal and spatial resolution. The essential components of the optogenetic toolbox consist of two kinds of molecular devices: actuators and reporters, which respectively enable light-mediated control or monitoring of molecular processes. The first generation of genetically encoded calcium reporters, fluorescent proteins, and neural activators has already had a great impact on neuroscience. Now, a second generation of voltage reporters, neural silencers, and functionally extended fluorescent proteins hold great promise for continuing this revolution. In this review, we will evaluate and highlight the limitations of presently available optogenic tools and discuss where these technologies and their applications are headed in the future.

Item Details

Item Type:Substantial Review
Keywords:Optogenetics, neuroscience
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Neurosciences not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
ID Code:97391
Year Published:2010
Web of Science® Times Cited:81
Deposited By:Medicine
Deposited On:2014-12-15
Last Modified:2014-12-15

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