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An Optimized Triple Modality Reporter for Quantitative In Vivo Tumor Imaging and Therapy Evaluation

Citation

Levin, RA and Felsen, CN and Yang, J and Lin, J and Whitney, MA and Nguyen, QT and Tsien, RY, An Optimized Triple Modality Reporter for Quantitative In Vivo Tumor Imaging and Therapy Evaluation, PL o S One, 9, (5) Article e97415. ISSN 1932-6203 (2014) [Refereed Article]


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Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) http://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1371/journal.pone.0097415

Abstract

We present an optimized triple modality reporter construct combining a far-red fluorescent protein (E2-Crimson), enhanced firefly luciferase enzyme (Luc2), and truncated wild type herpes simplex virus I thymidine kinase (wttk) that allows for sensitive, long-term tracking of tumor growth in vivo by fluorescence, bioluminescence, and positron emission tomography. Two human cancer cell lines (MDA-MB-231 breast cancer and HT-1080 fibrosarcoma cancer) were successfully transduced to express this triple modality reporter. Fluorescence and bioluminescence imaging of the triple modality reporter were used to accurately quantify the therapeutic responses of MDA-MB-231 tumors to the chemotherapeutic agent monomethyl auristatin E in vivo in athymic nude mice. Positive correlation was observed between the fluorescence and bioluminescence signals, and these signals were also positively correlated with the ex vivo tumor weights. This is the first reported use of both fluorescence and bioluminescence signals from a multi-modality reporter construct to measure drug efficacy in vivo.

Item Details

Item Type:Refereed Article
Keywords:Fluorescent reporter, cancer
Research Division:Technology
Research Group:Medical Biotechnology
Research Field:Medical Molecular Engineering of Nucleic Acids and Proteins
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Lin, J (Dr John Lin)
ID Code:97387
Year Published:2014
Web of Science® Times Cited:6
Deposited By:Medicine (Discipline)
Deposited On:2014-12-15
Last Modified:2017-11-06
Downloads:447 View Download Statistics

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