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Optogenetic control of Drosophila using a red-shifted channelrhodopsin reveals experience-dependent influences on courtship

Citation

Inagaki, HK and Jung, Y and Hoopfer, ED and Wong, AM and Mishra, N and Lin, J and Tsien, RY and Anderson, DJ, Optogenetic control of Drosophila using a red-shifted channelrhodopsin reveals experience-dependent influences on courtship, Nature Methods: techniques for life scientists and chemists, 11 pp. 325-332. ISSN 1548-7091 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Nature America, Inc.

DOI: doi:10.1038/nmeth.2765

Abstract

Optogenetics allows the manipulation of neural activity in freely moving animals with millisecond precision, but its application in Drosophila melanogaster has been limited. Here we show that a recently described red activatable channelrhodopsin (ReaChR) permits control of complex behavior in freely moving adult flies, at wavelengths that are not thought to interfere with normal visual function. This tool affords the opportunity to control neural activity over a broad dynamic range of stimulation intensities. Using time-resolved activation, we show that the neural control of male courtship song can be separated into (i) probabilistic, persistent and (ii) deterministic, command-like components. The former, but not the latter, neurons are subject to functional modulation by social experience, which supports the idea that they constitute a locus of state-dependent influence. This separation is not evident using thermogenetic tools, a result underscoring the importance of temporally precise control of neuronal activation in the functional dissection of neural circuits in Drosophila.

Item Details

Item Type:Refereed Article
Keywords:Optogenetics, neuroscience, drosophila
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Neurosciences not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Lin, J (Dr John Lin)
ID Code:97386
Year Published:2014 (online first 2013)
Deposited By:Medicine (Discipline)
Deposited On:2014-12-15
Last Modified:2015-05-20
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