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Optogenetic Inhibition of Synaptic Release with Chromophore-Assisted Light Inactivation (CALI)


Lin, J and Sann, SB and Zhou, K and Nabavi, S and Proulx, CD and Malinow, R and Jin, Y and Tsien, RY, Optogenetic Inhibition of Synaptic Release with Chromophore-Assisted Light Inactivation (CALI), Neuron, 79, (2) pp. 241-253. ISSN 0896-6273 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 Elsevier

DOI: doi:10.1016/j.neuron.2013.05.022


Optogenetic techniques provide effective ways of manipulating the functions of selected neurons with light. In the current study, we engineered an optogenetic technique that directly inhibits neurotransmitter release. We used a genetically encoded singlet oxygen generator, miniSOG, to conduct chromophore assisted light inactivation (CALI) of synaptic proteins. Fusions of miniSOG to VAMP2 and synaptophysin enabled disruption of presynaptic vesicular release upon illumination with blue light. In cultured neurons and hippocampal organotypic slices, synaptic release was reduced up to 100%. Such inhibition lasted >1 hr and had minimal effects on membrane electrical properties. When miniSOG-VAMP2 was expressed panneuronally in Caenorhabditis elegans, movement of the worms was reduced after illumination, and paralysis was often observed. The movement of the worms recovered overnight. We name this technique Inhibition of Synapses with CALI (InSynC). InSynC is a powerful way to silence genetically specified synapses with light in a spatially and temporally precise manner.

Item Details

Item Type:Refereed Article
Keywords:Optogenetics, neuroscience
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Neurosciences not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Lin, J (Dr John Lin)
ID Code:97384
Year Published:2013
Web of Science® Times Cited:126
Deposited By:Medicine
Deposited On:2014-12-15
Last Modified:2017-11-06

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