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ReaChR: a red-shifted variant of channelrhodopsin enables deep transcranial optogenetic excitation

Citation

Lin, J and Knutsen, PM and Muller, A and Kleinfeld, D and Tsien, RY, ReaChR: a red-shifted variant of channelrhodopsin enables deep transcranial optogenetic excitation, Nature Neuroscience, 16 pp. 1499-1508. ISSN 1097-6256 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 Nature America, Inc

DOI: doi:10.1038/nn.3502

Abstract

Channelrhodopsins (ChRs) are used to optogenetically depolarize neurons. We engineered a variant of ChR, denoted red-activatable ChR (ReaChR), that is optimally excited with orange to red light (λ ~590630 nm) and offers improved membrane trafficking, higher photocurrents and faster kinetics compared to existing red-shifted ChRs. Red light is less scattered by tissue and is absorbed less by blood than the blue to green wavelengths that are required by other ChR variants. We used ReaChR expressed in the vibrissa motor cortex to drive spiking and vibrissa motion in awake mice when excited with red light through intact skull. Precise vibrissa movements were evoked by expressing ReaChR in the facial motor nucleus in the brainstem and illumination with red light through the external auditory canal. Thus, ReaChR enables transcranial optical activation of neurons in deep brain structures without the need to surgically thin the skull, form a transcranial window or implant optical fibers.

Item Details

Item Type:Refereed Article
Keywords:optogenetics, neuroscience
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Neurosciences not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Lin, J (Dr John Lin)
ID Code:97383
Year Published:2013
Deposited By:Medicine (Discipline)
Deposited On:2014-12-15
Last Modified:2015-03-12
Downloads:0

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