University of Tasmania
Browse

File(s) not publicly available

Exacerbation of influenza infection by low doses of geogenic dust

conference contribution
posted on 2023-05-24, 13:17 authored by Clifford, HD, Perks, K, Larcombe, AN, Graeme ZoskyGraeme Zosky
Aim: To determine the effects of geogenic (earth-derived) PM10 (particulate matter <10 μm diameter) on pulmonary infl ammation and lung mechanics, and how this contributes to the exacerbation of a respiratory viral infection. Methods: Geogenic dust was directly sampled from the community of Karratha in the north of Western Australia, and the PM10 fraction was extracted. Adult female BALB/c mice were exposed to low doses of PM10 (10 μg per day for 10 days) by intranasal instillation (in 50 μL of saline + 0.05% Tween-80), while control mice received vehicle alone. Mice were infected with infl uenza (A/Mem/1/71) virus (or control VP-SFM media alone) at day 6, with lung function measured on day 11. Lung volume and mechanics were measured using plethysmography and a modifi cation of the forced oscillation technique (FOT). Mice were euthanased and bronchoalveolar lavage fl uid was collected for assessment of infl ammation (total and differential cell counts). Results: Infl uenza infection induced an infl ammatory response in the lung (infl ux of total infl ammatory cells p < 0.001; neutrophils p < 0.001). Geogenic PM10 from Karratha produced an additive effect on the infl ammatory response to infl uenza, with mice exposed to both having signifi cantly higher neutrophilia compared to those exposed to either insult alone (p = 0.003). Particle exposure signifi cantly impaired lung mechanics, with an increase in airway resistance (Raw, p < 0.001). Furthermore, exposure to PM10 synergistically impaired lung function during infl uenza infection (Raw, p < 0.001). Conclusion: Geogenic dust particles impair lung function and exacerbate the response to respiratory viral infection. This has important implications for respiratory health in communities exposed to high particulate loads of geogenic origin, such as those in the remote, arid regions of Australia.

History

Publication title

Respirology

Volume

18 (Supp. 2)

Editors

Bardin, P

Pagination

21

ISSN

1323-7799

Department/School

Tasmanian School of Medicine

Publisher

Wiley-Blackwell Publishing Asia

Place of publication

Australia

Event title

The Thoracic Society of Australia and New Zealand Annual Scientific Meeting 2013

Event Venue

Darwin, Australia

Date of Event (Start Date)

2013-03-23

Date of Event (End Date)

2013-03-27

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

Usage metrics

    University Of Tasmania

    Keywords

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC