97119 journal article.pdf (1.3 MB)
Modulation of cytokine production by drugs with antiepileptic or mood stabilizer properties in anti-CD3- and anti-CD40-stimulated blood in vitro
journal contribution
posted on 2023-05-18, 05:38 authored by Himmerich, H, Bartsch, S, Hamer, H, Mergl, R, Schonherr, J, Petersein, C, Munzer, A, Kenneth KirkbyKenneth Kirkby, Bauer, K, Sack, UIncreased cytokine production possibly due to oxidative stress has repeatedly been shown to play a pivotal role in the pathophysiology of epilepsy and bipolar disorder. Recent in vitro and animal studies of valproic acid (VPA) report antioxidative and anti-inflammatory properties, and suppression of interleukin (IL)-6 and tumor necrosis factor (TNF)-α. We tested the effect of drugs with antiepileptic or mood stabilizer properties, namely, primidone (PRM), carbamazepine (CBZ), levetiracetam (LEV), lamotrigine (LTG), VPA, oxcarbazepine (OXC), topiramate (TPM), phenobarbital (PB), and lithium on the production of the following cytokines in vitro: interleukin (IL)-1β, IL-2, IL-4, IL-6, IL-17, IL-22, and TNF-α. We performed a whole blood assay with stimulated blood of 14 healthy female subjects. Anti-human CD3 monoclonal antibody OKT3, combined with 5C3 antibody against CD40, was used as stimulant. We found a significant reduction of IL-1 and IL-2 levels with all tested drugs other than lithium in the CD3/5C3-stimulated blood; VPA led to a decrease in IL-1β, IL-2, IL-4, IL-6, IL-17, and TNF-α production, which substantiates and adds knowledge to current hypotheses on VPA’s anti-inflammatory properties.
History
Publication title
Oxidative Medicine and Cellular LongevityArticle number
806162Number
806162Pagination
1-11ISSN
1942-0900Department/School
Tasmanian School of MedicinePublisher
Hindawi Publishing CorporationPlace of publication
United StatesRights statement
Copyright 2014 Hubertus Himmerich et al. Licenced under Creative Commons Attribution 3.0 Unported (CC BY 3.0) http://creativecommons.org/licenses/by/3.0/Repository Status
- Open