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Microencapsulation of a putative probiotic Enterobacter species, C6-6, to protect rainbow trout, Oncorhynchus mykiss (Walbaum), against bacterial coldwater disease

Citation

Ghosh, B and Cain, KD and Nowak, BF and Bridle, AR, Microencapsulation of a putative probiotic Enterobacter species, C6-6, to protect rainbow trout, Oncorhynchus mykiss (Walbaum), against bacterial coldwater disease, Journal of Fish Diseases, 39, (1) pp. 1-11. ISSN 0140-7775 (2016) [Refereed Article]

Copyright Statement

Copyright 2014 John Wiley & Sons Ltd

DOI: doi:10.1111/jfd.12311

Abstract

Flavobacterium psychrophilum is the causative agent of bacterial coldwater disease (BCWD), which has a major impact on salmonid aquaculture globally. An Enterobacter species, C6-6, isolated from the gut of rainbow trout, Oncorhynchus mykiss (Walbaum), has been identified as a potential probiotic species providing protection against BCWD. This study examined the effects of alginate microencapsulation on the protective efficacy of C6-6 against BCWD in vivo when administered to rainbow trout fry orally or by intraperitoneal (IP) injection. Viable C6-6 bacteria were microencapsulated successfully, and this process (microencapsulation) did not significantly deteriorate its protective properties as compared to the administration of non-microencapsulated C6-6 bacteria. Both oral and IP delivery of C6-6 achieved significantly better protection than control treatments that did not contain C6-6 bacteria. The highest relative percent survival (RPS) resulted from IP delivery (71.4%) and was significantly greater than the highest oral RPS (38.6%). Successful intestinal colonization was not critical to protective effects of C6-6. The study showed that C6-6 administration, with or without encapsulation, was a viable choice for protecting fry from BCWD especially when administered intraperitoneally.

Item Details

Item Type:Refereed Article
Keywords:coldwater disease, disease control, Flavobacterium psychrophilum, microencapsulation, oral delivery, probiotic
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Fin Fish (excl. Tuna)
Author:Ghosh, B (Dr Bikramjit Ghosh)
Author:Nowak, BF (Professor Barbara Nowak)
Author:Bridle, AR (Dr Andrew Bridle)
ID Code:96882
Year Published:2016
Web of Science® Times Cited:4
Deposited By:Centre for Fisheries and Aquaculture
Deposited On:2014-11-25
Last Modified:2017-11-03
Downloads:0

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