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Administration of recombinant attachment protein (r22C03) of Neoparamoeba perurans induces humoral immune response against the parasite in Atlantic salmon (Salmo salar)

Citation

Valdenegro Vega, VA and Crosbie, PBB and Cook, MT and Vincent, BN and Nowak, BF, Administration of recombinant attachment protein (r22C03) of Neoparamoeba perurans induces humoral immune response against the parasite in Atlantic salmon (Salmo salar), Fish and Shellfish Immunology, 38, (2) pp. 294-302. ISSN 1050-4648 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Elsevier

DOI: doi:10.1016/j.fsi.2014.03.034

Abstract

This study investigated the use of a recombinant protein of Neoparamoeba perurans, the causative agent of Amoebic gill disease (AGD), as an immunogen to generate systemic and mucosal antibody responses against the parasite. Genes encoding N. perurans homologs of mannose-binding protein (MBP) from Acanthamoeba spp. have been identified. From these, a Neoparamoeba MBP – like EST has been identified and produced as a recombinant fusion protein. Attachment of N. perurans to the gill might be reduced by antibody-mediated interference of this protein, but this is dependent on the presence and level of functional antibodies in the mucus. Fish were immunized with the protein via i.p. injection with Freund's complete adjuvant (FCA); and serum and skin mucus samples were collected before and after immunization. Antibodies (IgM) present in samples were characterized via Western blot and their levels measured with an ELISA. The immunization was able to induce a systemic IgM response 8 weeks after primary exposure and a mucosal response 4 weeks post initial immunization, which were specific to the recombinant protein but not to antigens obtained from crude amoebic preparations. However, adherence of the antibodies to the parasite was observed using immunocytochemistry, and both, serum and skin mucus IgM, were able to bind the surface of formalin-fixed N. perurans. This finding may contribute to further research into the development of a vaccine for AGD.

Item Details

Item Type:Refereed Article
Keywords:amoebic gill disease, N. perurans, recombinant protein, systemic and mucosal antibodies
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Fin Fish (excl. Tuna)
Author:Valdenegro Vega, VA (Miss Victoria Valdenegro)
Author:Crosbie, PBB (Dr Philip Crosbie)
Author:Vincent, BN (Miss Benita Vincent)
Author:Nowak, BF (Professor Barbara Nowak)
ID Code:96857
Year Published:2014
Web of Science® Times Cited:1
Deposited By:NC Marine Conservation and Resource Sustainability
Deposited On:2014-11-24
Last Modified:2017-11-04
Downloads:0

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