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Support for the coevolution of Neoparamoeba and their endosymbionts, Perkinsela amoebae-like organisms

Citation

Young, ND and Dykova, I and Crosbie, PBB and Wolf, M and Morrison, RN and Bridle, AR and Nowak, BF, Support for the coevolution of Neoparamoeba and their endosymbionts, Perkinsela amoebae-like organisms, European Journal of Protistology, 50, (5) pp. 509-523. ISSN 0932-4739 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Elsevier

DOI: doi:10.1016/j.ejop.2014.07.004

Abstract

Some of the species from the genus Neoparamoeba, for example N. perurans have been shown to be pathogenic to aquatic animals and thus have economic significance. They all contain endosymbiont, Perkinsela amoebae like organisms (PLOs). In this study we investigated phylogenetic ambiguities within the Neoparamoeba taxonomy and phylogenetic congruence between PLOs and their host Neoparamoeba to confirm the existence of a single ancient infection/colonisation that led to cospeciation between all PLOs and their host Neoparamoeba. DNA was extracted and rRNA genes from host amoeba and endosymbiont were amplified using PCR. Uncertainties in the Neoparamoeba phylogeny were initially resolved by a secondary phylogenetic marker, the internal transcribed spacer 2 (ITS2). The secondary structure of ITS2 was reconstructed for Neoparamoeba. The ITS2 was phylogenetically informative, separating N. pemaquidensis and N. aestuarina into distinct monophyletic clades and designating N. perurans as the most phylogenetically divergent Neoparamoeba species. The new phylogenetic data were used to verify the tree topologies used in cophylogenetic analyses that revealed strict phylogenetic congruence between endosymbiotic PLOs with their host Neoparamoeba. Strict congruence in the phylogeny of all PLOs and their host Neoparamoeba was demonstrated implying that PLOs are transmitted vertically from parent to daughter cell.

Item Details

Item Type:Refereed Article
Keywords:endosymbiont, ITS2, Neoparamoeba, Perkinsela, phylogeny
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Young, ND (Mr Neil Young)
Author:Crosbie, PBB (Dr Philip Crosbie)
Author:Morrison, RN (Dr Richard Morrison)
Author:Bridle, AR (Dr Andrew Bridle)
Author:Nowak, BF (Professor Barbara Nowak)
ID Code:96856
Year Published:2014
Web of Science® Times Cited:8
Deposited By:NC Marine Conservation and Resource Sustainability
Deposited On:2014-11-24
Last Modified:2017-11-04
Downloads:0

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