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Persistent and compartmentalised disruption of dendritic cell subpopulations in the lung following Influenza A Virus infection

Citation

Strickland, DH and Fear, V and Shenton, S and Wikstrom, ME and Zosky, G and Larcombe, AN and Holt, PG and Berry, C and von Garnier, C and Stumbles, PA, Persistent and compartmentalised disruption of dendritic cell subpopulations in the lung following Influenza A Virus infection, PLoS One, 9, (11) Article e111520. ISSN 1932-6203 (2014) [Refereed Article]


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Copyright Statement

Copyright 2014 The Authors-This is an open-access article distributed under the terms of the Creative Commons Attribution License,(CC BY 4.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

DOI: doi:10.1371/journal.pone.0111520

Abstract

Immunological homeostasis in the respiratory tract is thought to require balanced interactions between networks of dendritic cell (DC) subsets in lung microenvironments in order to regulate tolerance or immunity to inhaled antigens and pathogens. Influenza A virus (IAV) poses a serious threat of long-term disruption to this balance through its potent pro-inflammatory activities. In this study, we have used a BALB/c mouse model of A/PR8/34 H1N1 Influenza Type A Virus infection to examine the effects of IAV on respiratory tissue DC subsets during the recovery phase following clearance of the virus. In adult mice, we found differences in the kinetics and activation states of DC residing in the airway mucosa (AMDC) compared to those in the parenchymal lung (PLDC) compartments. A significant depletion in the percentage of AMDC was observed at day 4 post-infection that was associated with a change in steady-state CD11b+ and CD11b AMDC subset frequencies and significantly elevated CD40 and CD80 expression and that returned to baseline by day 14 post-infection. In contrast, percentages and total numbers of PLDC were significantly elevated at day 14 and remained so until day 21 post-infection. Accompanying this was a change in CD11b+and CD11b PLDC subset frequencies and significant increase in CD40 and CD80 expression at these time points. Furthermore, mice infected with IAV at 4 weeks of age showed a significant increase in total numbers of PLDC, and increased CD40 expression on both AMDC and PLDC, when analysed as adults 35 days later. These data suggest that the rate of recovery of DC populations following IAV infection differs in the mucosal and parenchymal compartments of the lung and that DC populations can remain disrupted and activated for a prolonged period following viral clearance, into adulthood if infection occurred early in life.

Item Details

Item Type:Refereed Article
Keywords:dendritic cells, influenza, lung
Research Division:Medical and Health Sciences
Research Group:Cardiorespiratory Medicine and Haematology
Research Field:Respiratory Diseases
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Respiratory System and Diseases (incl. Asthma)
Author:Zosky, G (Associate Professor Graeme Zosky)
ID Code:96740
Year Published:2014
Web of Science® Times Cited:3
Deposited By:Medicine (Discipline)
Deposited On:2014-11-18
Last Modified:2017-11-01
Downloads:92 View Download Statistics

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