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Prevalence of Acanthamoeba spp. in Tasmanian intensive care clinical specimens

Citation

Bradbury, RS and French, LP and Blizzard, L, Prevalence of Acanthamoeba spp. in Tasmanian intensive care clinical specimens, Journal of Hospital Infection, 86, (3) pp. 178-181. ISSN 0195-6701 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 The Healthcare Infection Society

DOI: doi:10.1016/j.jhin.2013.12.009

Abstract

BACKGROUND: Acanthamoebae are ubiquitous free-living environmental amoebae that may occasionally cause keratitis, granulomatous encephalitis, cutaneous lesions and systemic disease in humans. Acanthamoeba spp. have been implicated as a vehicle by which a number of common bacterial causes of healthcare-associated pneumonia may enter the lungs. Limited evidence has been found implicating Acanthamoeba spp. as a primary cause of pneumonia and urinary catheter colonization in intensive care patients.

AIM: To explore the possibility of colonization of the respiratory and urinary tracts of intensive care patients with free-living amoebae.

METHODS: Thirty-nine catheter urines, 50 endotracheal trap sputa and one general ward sputum sample from 45 patients and nine intensive care unit (ICU) environmental water samples were collected during a four-and-half-month period in the Royal Hobart Hospital from August 2011.

FINDINGS: Acanthamoebae were isolated by culture and detected by polymerase chain reaction in two sputum samples from a single patient, taken one week apart. A single Acanthamoeba species isolate was detected by culture only from the ICU environment.

CONCLUSION: Colonization of ICU patients' respiratory tracts with Acanthamoeba spp. does occur. This may have significance for the role of acanthamoebae as a source of bacterial pathogens in intensive therapy patients' respiratory tracts.

Item Details

Item Type:Refereed Article
Keywords:Acanthamoeba; Healthcare-acquired infection; Intensive care; Nosocomial; Respiratory; Urinary
Research Division:Medical and Health Sciences
Research Group:Cardiorespiratory Medicine and Haematology
Research Field:Respiratory Diseases
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Respiratory System and Diseases (incl. Asthma)
Author:Bradbury, RS (Dr Richard Bradbury)
Author:Blizzard, L (Associate Professor Leigh Blizzard)
ID Code:96621
Year Published:2014
Web of Science® Times Cited:3
Deposited By:Menzies Institute for Medical Research
Deposited On:2014-11-13
Last Modified:2017-11-03
Downloads:0

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