We have developed novel device where a localised axonal stretch injury is achieved by the integration of a pressure-flexible valve in the axon compartment of a fluidically isolated neuron culture device. Defined stretch injuries applied using this device resulted in delayed neuronal alterations typical of those observed in vivo. These results confirm the suitability of this system for studying the neuronal response to axonal stretch injury. Therefore, this system provides a valuable tool for studying the degenerative and regenerative neuronal responses induced by acute stretch injury and provides a suitable platform for testing potential therapeutic agents.

Item Type:	Conference Extract
Keywords:	microfluidic, lab on a chip, axon stretch injury, PDMS device for cell culture, neuroscience, neuron culture, traumatic brain injury
Research Division:	Biomedical and Clinical Sciences
Research Group:	Neurosciences
Research Field:	Neurosciences not elsewhere classified
Objective Division:	Health
Objective Group:	Clinical health
Objective Field:	Clinical health not elsewhere classified
UTAS Author:	Yap, YC (Dr Yiing Yap)
UTAS Author:	Dickson, TC (Professor Tracey Dickson)
UTAS Author:	King, AE (Professor Anna King)
UTAS Author:	Breadmore, MC (Professor Michael Breadmore)
UTAS Author:	Guijt, RM (Dr Rosanne Guijt)
ID Code:	96620
Year Published:	2012
Deposited By:	Pharmacy
Deposited On:	2014-11-13
Last Modified:	2022-06-29
Downloads:	0