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Exploration of Phoslock® clay in mitigating Prymnesium parvum fish-killing algal blooms in aquaculture ponds
Citation
Seger, A and Body, A and Hallegraeff, G, Exploration of Phoslock clay in mitigating Prymnesium parvum fish-killing algal blooms in aquaculture ponds, Proceedings of the 15th International Conference on Harmful Algae, 29 October - 2 November 2012, Changwon, Korea, pp. 231-234. ISBN 978-87-990827-4-2 (2014) [Refereed Conference Paper]
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Abstract
When in 2009 a tropical Australian barramundi farm suffered from fish-killing Prymnesium parvum blooms,
the farm manager decided to manipulate N:P nutrients by adding Phoslock® bentonite clay and adjusting pH
through the addition of molasses. Phosphate levels were maintained at <0.03 mg/L, phytoplankton biomass
and dissolved oxygen became more stable and Prymnesium disappeared from the system altogether. We
conducted Prymnesium culture experiments under different N:P ratios to interpret field observations, while
measuring toxins using the RTgill-Wl cell line assay. Methanol extracts of nitrogen deficient cells were the
most toxic during stationary phase, followed by phosphorus deficient cells and with nutrient sufficient
cultures least toxic (7, 20, 65% gill cell viability, respectively). Sonicated whole-cell cultures were more
toxic after 4h (0-13% gill cell viability) but lost significant toxicity after 24h in the dark, suggesting toxin
degradation. Phoslock® effectively removed 60-100% of water soluble toxicity of live Prymnesium and
offers great potential as on-farm emergency response. We are currently refining clay type and dosage,
including exploration of application of clays to other ichthyotoxic algae.
Item Details
| Item Type: | Refereed Conference Paper |
|---|---|
| Keywords: | clay, flocculation, algal blooms |
| Research Division: | Biological Sciences |
| Research Group: | Plant Biology |
| Research Field: | Phycology (incl. Marine Grasses) |
| Objective Division: | Animal Production and Animal Primary Products |
| Objective Group: | Fisheries - Aquaculture |
| Objective Field: | Fisheries - Aquaculture not elsewhere classified |
| Author: | Seger, A (Mr Andreas Seger) |
| Author: | Hallegraeff, G (Professor Gustaaf Hallegraeff) |
| ID Code: | 96380 |
| Year Published: | 2014 |
| Funding Support: | Australian Research Council (DP130102859) |
| Deposited By: | IMAS Research and Education Centre |
| Deposited On: | 2014-11-03 |
| Last Modified: | 2016-06-08 |
| Downloads: | 1 View Download Statistics |
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