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Ultrastructural in situ hybridization and autoradiographic detection of foreign DNA in zebrafish spermatozoa

Citation

Patil, JG and Khoo, HW, Ultrastructural in situ hybridization and autoradiographic detection of foreign DNA in zebrafish spermatozoa, Biochemistry and Molecular Biology International, 35, (5) pp. 965-969. ISSN 1039-9712 (1995) [Refereed Article]

Abstract

Conditions have been established which permit specific hybridization of foreign DNA to complimentary DNA in ultra thin sections of zebrafish sperm prepared for electron microscopy (EM) after fixation in 2% paraformaldehyde, 2.5% glutaraldehyde and embedded in spurs resin. Zebrafish sperm incubated with foreign DNA were subjected to ultrastructural in situ hybridization and autoradiographic detection to determine if the sperm could retain and internalize the foreign DNA sequences. Specific and intense signals were detected from the sperm head indicating that the zebrafish sperm are capable of internalizing foreign DNA.

Item Details

Item Type:Refereed Article
Keywords:complementary dna; dna; animal cell; animal tissue; article; autoradiography; cell ultrastructure; dna determination; electron microscopy; in situ hybridization; internalization; male; nonhuman; spermatozoon; tissue fixation; zebra fish; Animals
Research Division:Biological Sciences
Research Group:Zoology
Research Field:Animal Cell and Molecular Biology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Patil, JG (Dr Jawahar Patil)
ID Code:96255
Year Published:1995
Web of Science® Times Cited:5
Deposited By:NC Ports and Shipping
Deposited On:2014-10-28
Last Modified:2014-10-28
Downloads:0

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