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Specific Detection of Pacific Oyster (Crassostrea gigas) Larvae in Plankton Samples Using Nested Polymerase Chain Reaction

Citation

Patil, JG and Gunasekera, RM and Deagle, BE and Bax, NJ, Specific Detection of Pacific Oyster (Crassostrea gigas) Larvae in Plankton Samples Using Nested Polymerase Chain Reaction, Marine Biotechnology, 7 pp. 11-20. ISSN 1436-2228 (2005) [Refereed Article]

Copyright Statement

Copyright 2005 Springer

DOI: doi:10.1007/s10126-004-0034-z

Abstract

Management of sustainable Pacific oyster fisheries would be assisted by an early, rapid, and accurate means of detecting their planktonic larvae. Reported here is an approach, based on polymerase chain reaction (PCR), for the detection of Pacific oyster larvae in plankton samples. Species-specific primers were designed by comparing partial mitochondrial cytochrome oxidase subunit I (COI) sequences from Crassostrea gigas, with other members of the family Ostreidae including those of Crassostrea angulata. Assay specificity was empirically validated through screening DNA samples obtained from several species of oysters. The assay was specific as only C. gigas samples returned PCR-positive results. A nested PCR approach could consistently detect 5 or more D-hinge-stage larvae spiked into a background of about 146 mg of plankton. The assay does not require prior sorting of larvae. We conclude that the assay could be used to screen environmental and ballast water samples, although further specificity testing against local bivalve species is recommended in new locations.

Item Details

Item Type:Refereed Article
Keywords:Crassostrea gigas, D-hinge, COI, nested PCR, introduced pest
Research Division:Technology
Research Group:Environmental Biotechnology
Research Field:Environmental Biotechnology Diagnostics (incl. Biosensors)
Objective Division:Environment
Objective Group:Control of Pests, Diseases and Exotic Species
Objective Field:Border Biosecurity (incl. Quarantine and Inspection)
Author:Patil, JG (Dr Jawahar Patil)
Author:Deagle, BE (Dr Bruce Deagle)
ID Code:96242
Year Published:2005
Web of Science® Times Cited:21
Deposited By:NC Ports and Shipping
Deposited On:2014-10-28
Last Modified:2014-11-10
Downloads:0

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