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Development and evaluation of a PCR based assay for detection of the toxic dinoflagellate, Gymnodinium catenatum (Graham) in ballast water and environmental samples

Citation

Patil, JG and Gunasekera, RM and Deagle, BE and Bax, NJ and Blackburn, SI, Development and evaluation of a PCR based assay for detection of the toxic dinoflagellate, Gymnodinium catenatum (Graham) in ballast water and environmental samples, Biological Invasions, 7 pp. 983-994. ISSN 1387-3547 (2005) [Refereed Article]

Copyright Statement

Copyright 2005 Springer

DOI: doi:10.1007/s10530-004-3119-8

Abstract

Gymnodinium catenatum is a bloom forming dinoflagellate that has been known to cause paralytic shell-fish poisoning (PSP) in humans. It is being reported with increased frequency around the world, with ballast water transport implicated as a primary vector that may have contributed to its global spread. Major limitations to monitoring and management of its spread are the inability for early, rapid, and accurate detection of G. catenatum in plankton samples. This study explored the feasibility of developing a PCR-based method for specific detection of G. catenatum in cultures and heterogeneous ballast water and environmental samples. Sequence comparison of the large sub unit (LSU) ribosomal DNA locus of several strains and species of dinoflagellates allowed the design of G. catenatum specific PCR primers that are flanked by conserved regions. Assay specificity was validated through screening a range of dino- flagellate cultures, including the morphologically similar and taxonomically closely related species G. nolleri. amplification of the diagnostic PCR product from all the strains of G. catenatum but not from other species of dinoflagellates tested imply the species specificity of the assay. Sensitivity of the assay to detect cysts in ballast water samples was established by simulated spiked experiments. The assay could detect G. catenatum in all ‘blank’ plankton samples that were spiked with five or more cysts. The assay was used to test environmental samples collected from the Derwent river estuary, Tasmania. Based on the results we conclude that the assay may be utilized in large scale screening of environmental and ballast water samples.

Item Details

Item Type:Refereed Article
Keywords:cyst, dinoflagellate, Gymnodinium catenatum, introduced pest, PCR detection
Research Division:Technology
Research Group:Environmental Biotechnology
Research Field:Environmental Biotechnology Diagnostics (incl. Biosensors)
Objective Division:Environment
Objective Group:Control of Pests, Diseases and Exotic Species
Objective Field:Border Biosecurity (incl. Quarantine and Inspection)
Author:Patil, JG (Dr Jawahar Patil)
ID Code:96237
Year Published:2005
Web of Science® Times Cited:19
Deposited By:NC Ports and Shipping
Deposited On:2014-10-28
Last Modified:2014-11-10
Downloads:0

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