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Centromere RNA is a key component for the assembly of nucleoproteins at the nucleolus and centromere
Citation
Wong, LH and Brettingham-Moore, KH and Chan, L and Quach, JM and Anderson, MA and Northrop, EL and Hannan, R and Saffery, R and Shaw, ML and Williams, E and Choo, KHA, Centromere RNA is a key component for the assembly of nucleoproteins at the nucleolus and centromere, Genome Research, 17, (8) pp. 1146-1160. ISSN 1088-9051 (2007) [Refereed Article]
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Licensed under Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0) http://creativecommons.org/licenses/by-nc/3.0/
Abstract
The centromere is a complex structure, the components and assembly pathway of which remain inadequately defined. Here, we demonstrate that centromeric α-satellite RNA and proteins CENPC1 and INCENP accumulate in the human interphase nucleolus in an RNA polymerase I-dependent manner. The nucleolar targeting of CENPC1 and INCENP requires α-satellite RNA, as evident from the delocalization of both proteins from the nucleolus in RNase-treated cells, and the nucleolar relocalization of these proteins following α-satellite RNA replenishment in these cells. Using protein truncation and in vitro mutagenesis, we have identified the nucleolar localization sequences on CENPC1 and INCENP. We present evidence that CENPC1 is an RNA-associating protein that binds α-satellite RNA by an in vitro binding assay. Using chromatin immunoprecipitation, RNase treatment, and "RNA replenishment" experiments, we show that α-satellite RNA is a key component in the assembly of CENPC1, INCENP, and survivin (an INCENP-interacting protein) at the metaphase centromere. Our data suggest that centromere satellite RNA directly facilitates the accumulation and assembly of centromere-specific nucleoprotein components at the nucleolus and mitotic centromere, and that the sequestration of these components in the interphase nucleolus provides a regulatory mechanism for their timely release into the nucleoplasm for kinetochore assembly at the onset of mitosis.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | centromere, RNA |
| Research Division: | Biomedical and Clinical Sciences |
| Research Group: | Medical biochemistry and metabolomics |
| Research Field: | Medical biochemistry - nucleic acids |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding knowledge |
| Objective Field: | Expanding knowledge in the biological sciences |
| UTAS Author: | Brettingham-Moore, KH (Dr Kate Brettingham-Moore) |
| ID Code: | 95837 |
| Year Published: | 2007 |
| Web of Science® Times Cited: | 213 |
| Deposited By: | Menzies Institute for Medical Research |
| Deposited On: | 2014-10-09 |
| Last Modified: | 2015-04-08 |
| Downloads: | 407 View Download Statistics |
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