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Visualisation of Zebrafish infection by GFP-labelled Vibrio anguillarum
Citation
O'Toole, RF and Von Hofsten, J and Rosqvist, R and Olsson, P-E and Wolf-Watz, H, Visualisation of Zebrafish infection by GFP-labelled Vibrio anguillarum, Microbial Pathogenesis, 37, (1) pp. 41-46. ISSN 0882-4010 (2004) [Refereed Article]
DOI: doi:10.1016/j.micpath.2004.03.001
Abstract
Vibrio anguillarum is an invasive pathogen of fish causing a septicaemia called vibriosis. In this work, transparent zebrafish were immersed in water containing green fluorescent protein labelled V. anguillarum. The infection was visualised at the whole fish and single bacterium levels using microscopy. The gastrointestinal tract was the first site where the pathogen was detected. This enteric localisation occurred independently of the flagellum or motility. On the other hand, chemotactic motility was essential for association of the pathogen with the fish surface. In conclusion, the zebrafish infection model provides evidence that the intestine and skin represent sites of infection by V. anguillarum and suggests a host site where chemotaxis may function in virulence.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | Chemotaxis; Flagellum; Green fluorescent protein; Vibriosis |
| Research Division: | Biological Sciences |
| Research Group: | Microbiology |
| Research Field: | Bacteriology |
| Objective Division: | Health |
| Objective Group: | Clinical health |
| Objective Field: | Clinical health not elsewhere classified |
| UTAS Author: | O'Toole, RF (Dr Ronan O'Toole) |
| ID Code: | 95789 |
| Year Published: | 2004 |
| Web of Science® Times Cited: | 122 |
| Deposited By: | Medicine |
| Deposited On: | 2014-10-08 |
| Last Modified: | 2014-10-08 |
| Downloads: | 0 |
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