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Visualisation of Zebrafish infection by GFP-labelled Vibrio anguillarum

Citation

O'Toole, RF and Von Hofsten, J and Rosqvist, R and Olsson, P-E and Wolf-Watz, H, Visualisation of Zebrafish infection by GFP-labelled Vibrio anguillarum, Microbial Pathogenesis, 37, (1) pp. 41-46. ISSN 0882-4010 (2004) [Refereed Article]

DOI: doi:10.1016/j.micpath.2004.03.001

Abstract

Vibrio anguillarum is an invasive pathogen of fish causing a septicaemia called vibriosis. In this work, transparent zebrafish were immersed in water containing green fluorescent protein labelled V. anguillarum. The infection was visualised at the whole fish and single bacterium levels using microscopy. The gastrointestinal tract was the first site where the pathogen was detected. This enteric localisation occurred independently of the flagellum or motility. On the other hand, chemotactic motility was essential for association of the pathogen with the fish surface. In conclusion, the zebrafish infection model provides evidence that the intestine and skin represent sites of infection by V. anguillarum and suggests a host site where chemotaxis may function in virulence.

Item Details

Item Type:Refereed Article
Keywords:Chemotaxis; Flagellum; Green fluorescent protein; Vibriosis
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Bacteriology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Infectious Diseases
Author:O'Toole, RF (Dr Ronan O'Toole)
ID Code:95789
Year Published:2004
Web of Science® Times Cited:92
Deposited By:Medicine (Discipline)
Deposited On:2014-10-08
Last Modified:2014-10-08
Downloads:0

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