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Modifying culture conditions in chemical library screening identifies alternative inhibitors of mycobacteria
journal contribution
posted on 2023-05-18, 04:21 authored by Miller, CH, Nisa, S, Dempsey, S, Jack, C, O'Toole, RFIn this study, application of a dual absorbance/fluorescence assay to a chemical library screen identified several previously unknown inhibitors of mycobacteria. In addition, growth conditions had a significant effect on the activity profile of the library. Some inhibitors such as Se-methylselenocysteine were detected only when screening was performed under nutrient-limited culture conditions as opposed to nutrient-rich culture conditions. We propose that multiple culture condition library screening is required for complete inhibitory profiling and for maximal antimycobacterial compound detection.
History
Publication title
Antimicrobial Agents and ChemotherapyVolume
53Issue
12Pagination
5279-5283ISSN
0066-4804Department/School
Tasmanian School of MedicinePublisher
Amer Soc MicrobiologyPlace of publication
1752 N St Nw, Washington, USA, Dc, 20036-2904Repository Status
- Restricted