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Modifying culture conditions in chemical library screening identifies alternative inhibitors of mycobacteria

Citation

Miller, CH and Nisa, S and Dempsey, S and Jack, C and O'Toole, RF, Modifying culture conditions in chemical library screening identifies alternative inhibitors of mycobacteria, Antimicrobial Agents and Chemotherapy, 53, (12) pp. 5279-5283. ISSN 0066-4804 (2009) [Refereed Article]

DOI: doi:10.1128/AAC.00803-09

Abstract

In this study, application of a dual absorbance/fluorescence assay to a chemical library screen identified several previously unknown inhibitors of mycobacteria. In addition, growth conditions had a significant effect on the activity profile of the library. Some inhibitors such as Se-methylselenocysteine were detected only when screening was performed under nutrient-limited culture conditions as opposed to nutrient-rich culture conditions. We propose that multiple culture condition library screening is required for complete inhibitory profiling and for maximal antimycobacterial compound detection.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Medical Microbiology
Research Field:Medical Bacteriology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Infectious Diseases
Author:O'Toole, RF (Dr Ronan O'Toole)
ID Code:95775
Year Published:2009
Web of Science® Times Cited:8
Deposited By:Medicine (Discipline)
Deposited On:2014-10-08
Last Modified:2014-10-08
Downloads:0

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