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A comparison of biogenic iron quotas during a diatom spring bloom using multiple approaches

Citation

King, AL and Sanudo-Wilhelmy, SA and Boyd, PW and Twining, BS and Wilhelm, SW and Breene, C and Ellwood, MJ and Hutchins, DA, A comparison of biogenic iron quotas during a diatom spring bloom using multiple approaches, Biogeosciences, 9, (2) pp. 667-687. ISSN 1726-4170 (2012) [Refereed Article]


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Licensed under Creative Commons Attribution 3.0 Unported (CC BY 3.0) http://creativecommons.org/licenses/by/3.0/

DOI: doi:10.5194/bg-9-667-2012

Abstract

Biogenic Fe quotas were determined using three distinct techniques on samples collected concurrently in the subtropical Pacific Ocean east of New Zealand. Fe quotas were measured using radioisotope uptake experiments (24 h incubation), bulk filtration and analysis by inductively-coupled plasma mass spectrometer (ICPMS), and single-cell synchrotron X-ray fluorescence (SXRF) analysis over a sixteen-day period (year days 263 to 278 of 2008) during a quasi-Lagrangian drifter experiment that tracked the evolution of the annual spring diatom bloom within a counter-clockwise open-ocean eddy. Overall, radioisotope uptake-determined Fe quotas (washed with oxalate reagent to remove extracellular Fe) were the lowest (0.5-1.0 mmol Fe:mol P; 4-8 μmol Fe:mol C), followed by single-cell Fe quotas (2.3-7.5 mmol Fe:mol P; 17-57 μmol Fe:mol C), and the highest and most variable quotas were from the bulk filtration ICPMS approach that used the oxalate reagent wash, corrected for lithogenic Fe using Al (0.8-21 mmol Fe:mol P; 4-136 μmol Fe:mol C). During the evolution of the spring bloom within the eddy (year days 263 to 272), the surface mixed layer inventories of particulate biogenic elements (C, N, P, Si) and chlorophyll increased while Fe quotas estimated from all three approaches exhibited a general decline. After the onset of the bloom decline, the drogued buoys exited the eddy center (days 273 to 277). Fe quotas returned to pre-bloom values during this part of the study. Our standardized and coordinated sampling protocols reveal the general observed trend in Fe quotas: ICPMS > SXRF > radioisotope uptake. We discuss the inherent differences between the techniques and argue that each technique has its individual merits and uniquely contributes to the characterization of the oceanic particulate Fe pool.

Item Details

Item Type:Refereed Article
Keywords:algal bloom, biogenic deposit, biological uptake, comparative study, diatom, eddy covariance, filtration, iron, mass spectrometry, pollution effect, radionuclide, X-ray fluorescence
Research Division:Biological Sciences
Research Group:Ecology
Research Field:Marine and Estuarine Ecology (incl. Marine Ichthyology)
Objective Division:Environment
Objective Group:Climate and Climate Change
Objective Field:Climate Change Adaptation Measures
Author:Boyd, PW (Professor Philip Boyd)
ID Code:95564
Year Published:2012
Web of Science® Times Cited:17
Deposited By:IMAS Research and Education Centre
Deposited On:2014-10-03
Last Modified:2017-11-01
Downloads:231 View Download Statistics

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