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Novel antioxidative peptides from the protein hydrolysate of oysters (Crassostrea talienwhanensis)
Citation
Wang, Q and Li, W and He, Y and Ren, D and Kow, F and Song, L and Yu, X, Novel antioxidative peptides from the protein hydrolysate of oysters (Crassostrea talienwhanensis), Food Chemistry, 145 pp. 991-996. ISSN 0308-8146 (2014) [Refereed Article]
Copyright Statement
Copyright 2014 Elsevier
DOI: doi:10.1016/j.foodchem.2013.08.099
Abstract
The antioxidative activity of hydrolysate peptides from oysters (Crassostrea talienwhanensis) was investigated.
After hydrolysis with subtilisin, the yields of the peptides that were soluble in trichloroacetic acid
(TCA-soluble) and the antioxidant activities of the resulting hydrolysate were determined using an
orthogonal design and a hydroxyl radical scavenging reaction. The hydrolysate was fractionated using
Sephadex G-15 gel filtration chromatography, and the two resulting bioactive peptides were subsequently
purified by RP-HPLC with a Kromasil C18 (ODS) column. The amino acid sequences were analyzed
by nano-ESI-MS/MS.
The critical reaction temperature, pH, hydrolysis time and enzyme-to-substrate (E/S) ratio were determined for the optimum hydrolysis with subtilisin, and the E/S ratio was found to be the most critical reaction condition. The amino acid sequences of the peptides (518 and 440 Da) were proline-valinemethionine- glycine-aspartic acid (PVMGA) and glutamine-histidine-glycine-valine (QHGV), respectively. These two novel peptides exhibited high antioxidative actions based on their hydroxyl and 1,1-diphenyl- 2-picrylhydrazyl (DPPH) radical scavenging activities.
Item Details
Item Type: | Refereed Article |
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Keywords: | antioxidative peptide, hydrolysate, oyster, Crassostrea talienwhanensis, radical scavenging activity, subtilisin |
Research Division: | Agricultural, Veterinary and Food Sciences |
Research Group: | Fisheries sciences |
Research Field: | Fisheries sciences not elsewhere classified |
Objective Division: | Animal Production and Animal Primary Products |
Objective Group: | Fisheries - aquaculture |
Objective Field: | Aquaculture oysters |
UTAS Author: | Kow, F (Dr Felicia Kow) |
ID Code: | 95307 |
Year Published: | 2014 |
Web of Science® Times Cited: | 60 |
Deposited By: | NC Marine Conservation and Resource Sustainability |
Deposited On: | 2014-09-30 |
Last Modified: | 2015-03-17 |
Downloads: | 0 |
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