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Novel antioxidative peptides from the protein hydrolysate of oysters (Crassostrea talienwhanensis)

Citation

Wang, Q and Li, W and He, Y and Ren, D and Kow, F and Song, L and Yu, X, Novel antioxidative peptides from the protein hydrolysate of oysters (Crassostrea talienwhanensis), Food Chemistry, 145 pp. 991-996. ISSN 0308-8146 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Elsevier

DOI: doi:10.1016/j.foodchem.2013.08.099

Abstract

The antioxidative activity of hydrolysate peptides from oysters (Crassostrea talienwhanensis) was investigated. After hydrolysis with subtilisin, the yields of the peptides that were soluble in trichloroacetic acid (TCA-soluble) and the antioxidant activities of the resulting hydrolysate were determined using an orthogonal design and a hydroxyl radical scavenging reaction. The hydrolysate was fractionated using Sephadex G-15 gel filtration chromatography, and the two resulting bioactive peptides were subsequently purified by RP-HPLC with a Kromasil C18 (ODS) column. The amino acid sequences were analyzed by nano-ESI-MS/MS.

The critical reaction temperature, pH, hydrolysis time and enzyme-to-substrate (E/S) ratio were determined for the optimum hydrolysis with subtilisin, and the E/S ratio was found to be the most critical reaction condition. The amino acid sequences of the peptides (518 and 440 Da) were proline-valinemethionine- glycine-aspartic acid (PVMGA) and glutamine-histidine-glycine-valine (QHGV), respectively. These two novel peptides exhibited high antioxidative actions based on their hydroxyl and 1,1-diphenyl- 2-picrylhydrazyl (DPPH) radical scavenging activities.

Item Details

Item Type:Refereed Article
Keywords:antioxidative peptide, hydrolysate, oyster, Crassostrea talienwhanensis, radical scavenging activity, subtilisin
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Fisheries Sciences not elsewhere classified
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Oysters
Author:Kow, F (Dr Felicia Kow)
ID Code:95307
Year Published:2014
Web of Science® Times Cited:19
Deposited By:NC Marine Conservation and Resource Sustainability
Deposited On:2014-09-30
Last Modified:2015-03-17
Downloads:0

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