File(s) under permanent embargo
Expression and knockdown of primordial germ cell genes, vasa, nanos and dead end in common Carp (Cyprinus carpio) embryos for transgenic sterilization and reduced sexual maturity
journal contribution
posted on 2023-05-18, 03:43 authored by Su, B, Peatman, E, Shang, M, Thresher, R, Grewe, P, Jawahar PATIL, Pinkert, CA, Irwin, MH, Li, C, Perera, DA, Duncan, PL, Fobes, M, Dunham, RACommon carp, Cyprinus carpio, is one of the most widely grown aquaculture species. Four types of knock-down constructs designed to transgenically sterilize fish were electroporated into common carp embryos to knockdown or eliminate expression of primordial germ cell (PGC) proteins, nanos or dead end. Embryos from different developmental stages were collected and tested using quantitative real-time PCR. Relative levels of gene expression were compared among control embryos and embryos exposed to these constructs during development. Two proteins, nanos and dead end, were targeted, each specific for a set of constructs, and a third related, but non-targeted PGC protein, vasa, was assayed. The effectiveness of the constructs varied depending upon the promoter. The expression levels of these proteins naturally decreased during development. In some, but not all cases, the expression of targeted mRNA was knocked down to minimal levels or degradation was accelerated. Up-regulation also was observed for dead end, possibly due to interferon responses. In other cases, the sterilization constructs had little effect on the targeted gene, but strongly down-regulated one or two of the alternative PGC genes examined in this study. At 19 months of age, common carp males exposed to these constructs had reduced rates of sexual maturity; however, reduction of female rates of sexual maturity was not conclusively shown. These constructs appear promising for transgenic sterilization of common carp.
History
Publication title
AquacultureVolume
420-421Issue
Supplement 1Pagination
S72-S84ISSN
0044-8486Department/School
Institute for Marine and Antarctic StudiesPublisher
Elsevier Science BvPlace of publication
Po Box 211, Amsterdam, Netherlands, 1000 AeRights statement
Copyright 2014 ElsevierRepository Status
- Restricted