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Cryopreservation of placental biopsies for mitochondrial respiratory analysis

Citation

Colleoni, F and Morash, AJ and Ashmore, T and Monk, M and Burton, GJ and Murray, AJ, Cryopreservation of placental biopsies for mitochondrial respiratory analysis, Placenta, 33, (2) pp. 122-123. ISSN 0143-4004 (2012) [Refereed Article]

DOI: doi:10.1016/j.placenta.2011.11.019

Abstract

Mitochondrial function is required to support energetically-demanding processes in the placenta. As such, a compromise in mitochondrial function could severely impact fetal growth and development. Respirometry is a highly useful method for studying mitochondrial function, but is not possible in freeze-thawed mitochondria, which become uncoupled. We have developed a novel method that permits respiratory analysis of cryopreserved placental tissue. We studied mitochondrial function in 7 normal human placentas, analysing both fresh and cryopreserved samples. We found no impairments in respiration following cryopreservation in the delivery suite, with enhanced coupling, as indicated by higher respiratory control ratios, than in fresh placental samples transported to the laboratory on ice.

Item Details

Item Type:Refereed Article
Keywords:metabolism, mitochondria, placenta, respiration
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Analytical Biochemistry
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Medical and Health Sciences
Author:Morash, AJ (Dr Andrea Morash)
ID Code:95040
Year Published:2012
Web of Science® Times Cited:6
Deposited By:Research Division
Deposited On:2014-09-23
Last Modified:2014-09-23
Downloads:0

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