94952 - BSA reduces inhibition in a TaqMan assay.pdf (87.58 kB)
BSA reduces inhibition in a TaqMan® assay for the detection of Batrachochytrium dendrobatidis
journal contribution
posted on 2023-05-18, 03:33 authored by Garland, S, Baker, A, Phillott, AD, Skerratt, LFA TaqMan® assay for the causative agent of chytridiomycosis in amphibians (Batrachochytrium dendrobatidis) can be inhibited by phenolic compounds, including humic and tannic acids, resulting in false negatives. Bovine serum albumin (BSA) is known to reduce inhibition of PCR when samples are contaminated with these inhibitors. We assessed the effect of BSA in reducing inhibition of the TaqMan® assay when analyzing skin swabs for B. dendrobatidis. We found that the addition of BSA to the TaqMan® reaction reduced inhibition to insignificant levels. BSA did not appreciably affect the efficiency or analytical sensitivity of the TaqMan® reaction in the analysis of standard DNA solutions free from environmental inhibitors. We recommend the addition of 400 ng µl–1 of BSA to the standard TaqMan® assay to reduce inhibition associated with sampling wild amphibians.
History
Publication title
Diseases of Aquatic OrganismsVolume
92Issue
2-3Pagination
113-116ISSN
0177-5103Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
Inter-ResearchPlace of publication
Nordbunte 23, Oldendorf Luhe, Germany, D-21385Rights statement
Copyright 2010 Inter-ResearchRepository Status
- Open