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Differences between deposited and soluble fibulin-1 in airway smooth muscle cells

Citation

Chen, L and Ge, Q and Black, JL and Burgess, JK and Oliver, BGG, Differences between deposited and soluble fibulin-1 in airway smooth muscle cells, Respirology, 23-27 March, 2013, Darwin, Australia, pp. 47. ISSN 1323-7799 (2013) [Conference Extract]


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Abstract

Introduction: Fibulin-1 (FBLN-1) is a secreted glycoprotein that is associated with extracellular matrix (ECM) formation and rebuilding. Altered deposition of the ECM is a hallmark of many fi brotic diseases, such as COPD where the airway thickness is increased.

Aim: To investigate the regulation of FBLN-1 in the presence of transforming growth factor beta 1 (TGF-β1) (a pro-fi brotic stimulus) in human airway smooth muscle (ASM) cells from COPD and non COPD volunteers.

Methods: ASM cells were plated at a density of 1 104 cells/cm2, and stimulated with or without TGF-β1 (10 ng/mL) for 72 h. Supernatant and cell free ECM were collected then the soluble and deposited FBLN-1 were measured by western blot and ELISA respectively. FBLN-1 mRNA fold change (during time course 4, 8, 24, 48, 72 h) was detected by real-time PCR.

Results: TGF-β1 decreased soluble FBLN-1 from human ASM cells isolated from both COPD and Non COPD volunteers (COPD n = 9, p ≤ 0.001, Non COPD n = 9, p ≤ 0.001), however, the deposition of FBLN-1 was increased (COPD n = 10, p ≤ 0.01, Non COPD n = 8, p ≤ 0.01). TGF-β1 did not increase FBLN-1 gene expression. There was no difference between cells from people with or without COPD.

Conclusions: The increased deposition of FBLN-1 in the ECM by TGF-β1 is likely due to incorporation of soluble FBLN-1 rather than de novo synthesis.

Item Details

Item Type:Conference Extract
Keywords:Fibulin-1; Extracellular Matrix; Airway Smooth Muscle; Transforming Growth Factor-beta 1
Research Division:Medical and Health Sciences
Research Group:Cardiorespiratory Medicine and Haematology
Research Field:Respiratory Diseases
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Respiratory System and Diseases (incl. Asthma)
Author:Chen, L (Dr Ling Chen)
ID Code:94872
Year Published:2013
Deposited By:Medicine (Discipline)
Deposited On:2014-09-18
Last Modified:2014-10-24
Downloads:0

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