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Probiont niche specialization contributes to additive protection against Vibrio owensii in spiny lobster larvae

Citation

Goulden, EF and Hall, MR and Pereg, LL and Baillie, BK and Hoj, L, Probiont niche specialization contributes to additive protection against Vibrio owensii in spiny lobster larvae, Environmental Microbiology Reports, 5, (1) pp. 39-48. ISSN 1758-2229 (2012) [Refereed Article]

Copyright Statement

Copyright 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.

DOI: doi:10.1111/1758-2229.12007

Abstract

The development of efficient probiotic application protocols for use in marine larviculture relies on comprehensive understanding of pathogen–probiont–host interactions. The probiont combination of Pseudoalteromonas sp. PP107 and Vibrio sp. PP05 provides additive protection against vectored Vibrio owensii DY05 infection in larvae (phyllosomas) of ornate spiny lobster, Panulirus ornatus. Here, fluorescently tagged strains were used to demonstrate niche specialization of these probionts in both the live feed vector organism Artemia and in phyllosomas. The pathogen was vulnerable to direct interaction with PP05 in the bacterioplankton as well as in the Artemia gut and the phyllosoma foregut and midgut gland. In contrast, PP107 was localized on external surfaces of Artemia and phyllosomas, and direct interaction with the pathogen was limited to the bacterioplankton. While PP107 was the overall dominant ectobiont on the phyllosoma cephalothorax and inner leg segments, PP05 was the primary colonizer of outer leg segments, nutrient-rich locales that may promote ingestion during feeding. This study shows that niche specialization can contribute to the additive probiotic effect of a probiotic mixture and highlights that probiotic enrichment of Artemia cultures can intercept the infection cycle of V. owensii DY05 in early-stage P. ornatus phyllosomas.

Item Details

Item Type:Refereed Article
Keywords:Panulirus ornatus, phyllosoma, Vibrio owensii, fluorescent protein, probiotics, niche specialisation
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Rock Lobster
Author:Goulden, EF (Dr Evan Goulden)
ID Code:94716
Year Published:2012
Web of Science® Times Cited:4
Deposited By:Sustainable Marine Research Collaboration
Deposited On:2014-09-16
Last Modified:2014-10-01
Downloads:0

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