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Probiont niche specialization contributes to additive protection against Vibrio owensii in spiny lobster larvae

journal contribution
posted on 2023-05-18, 03:21 authored by Evan Goulden, Hall, MR, Pereg, LL, Baillie, BK, Hoj, L
The development of efficient probiotic application protocols for use in marine larviculture relies on comprehensive understanding of pathogen–probiont–host interactions. The probiont combination of Pseudoalteromonas sp. PP107 and Vibrio sp. PP05 provides additive protection against vectored Vibrio owensii DY05 infection in larvae (phyllosomas) of ornate spiny lobster, Panulirus ornatus. Here, fluorescently tagged strains were used to demonstrate niche specialization of these probionts in both the live feed vector organism Artemia and in phyllosomas. The pathogen was vulnerable to direct interaction with PP05 in the bacterioplankton as well as in the Artemia gut and the phyllosoma foregut and midgut gland. In contrast, PP107 was localized on external surfaces of Artemia and phyllosomas, and direct interaction with the pathogen was limited to the bacterioplankton. While PP107 was the overall dominant ectobiont on the phyllosoma cephalothorax and inner leg segments, PP05 was the primary colonizer of outer leg segments, nutrient-rich locales that may promote ingestion during feeding. This study shows that niche specialization can contribute to the additive probiotic effect of a probiotic mixture and highlights that probiotic enrichment of Artemia cultures can intercept the infection cycle of V. owensii DY05 in early-stage P. ornatus phyllosomas.

History

Publication title

Environmental Microbiology Reports

Volume

5

Pagination

39-48

ISSN

1758-2229

Department/School

Institute for Marine and Antarctic Studies

Publisher

Wiley-Blackwell Publishing

Place of publication

United Kingdom

Rights statement

Copyright 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.

Repository Status

  • Restricted

Socio-economic Objectives

Aquaculture rock lobster

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