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Pathogenicity and infection cycle of Vibrio owensii in larviculture of the ornate spiny lobster (Panulirus ornatus)
Citation
Goulden, EF and Hall, MR and Bourne, DG and Pereg, LL and Hoj, L, Pathogenicity and infection cycle of Vibrio owensii in larviculture of the ornate spiny lobster (Panulirus ornatus), Applied and Environmental Microbiology, 78, (8) pp. 2841-2849. ISSN 0099-2240 (2012) [Refereed Article]
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Copyright Statement
Copyright 2012 American Society for Microbiology
Abstract
The type strain of Vibrio owensii (DY05) was isolated during an epizootic of aquaculture-reared larvae (phyllosomas) of the
ornate spiny lobster (Panulirus ornatus). V. owensii DY05 was formally demonstrated to be the etiological agent of a disease
causing rapid and reproducible larval mortality with pathologies similar to those seen during disease epizootics. Vectored challenge
via the aquaculture live feed organism Artemia (brine shrimp) caused consistent cumulative mortality rates of 84 to 89%
after 72 h, in contrast to variable mortality rates seen after immersion challenge. Histopathological examination of vector-challenged
phyllosomas revealed bacterial proliferation in the midgut gland (hepatopancreas) concomitant with epithelial cell necrosis.
A fluorescent-protein-labeled V. owensii DY05 transconjugant showed dispersal of single cells in the foregut and hepatopancreas
6 h postexposure, leading to colonization of the entire hepatopancreas within 18 h and eventually systemic infection. V.
owensii DY05 is a marine enteropathogen highly virulent to P. ornatus phyllosoma that uses vector-mediated transmission and
release from host association to a planktonic existence to perpetuate transfer. This understanding of the infection process will
improve targeted biocontrol strategies and enhance the prospects of commercially viable larviculture for this valuable spiny lobster
species.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | Panulirus ornatus, phyllosoma, Vibrio owensii, green fluorescent protein, infection cycle |
| Research Division: | Agricultural, Veterinary and Food Sciences |
| Research Group: | Fisheries sciences |
| Research Field: | Aquaculture |
| Objective Division: | Animal Production and Animal Primary Products |
| Objective Group: | Fisheries - aquaculture |
| Objective Field: | Aquaculture crustaceans (excl. rock lobster and prawns) |
| UTAS Author: | Goulden, EF (Dr Evan Goulden) |
| ID Code: | 94708 |
| Year Published: | 2012 |
| Web of Science® Times Cited: | 27 |
| Deposited By: | Sustainable Marine Research Collaboration |
| Deposited On: | 2014-09-16 |
| Last Modified: | 2014-10-13 |
| Downloads: | 327 View Download Statistics |
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