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Pathogenicity and infection cycle of Vibrio owensii in larviculture of the ornate spiny lobster (Panulirus ornatus)

Citation

Goulden, EF and Hall, MR and Bourne, DG and Pereg, LL and Hoj, L, Pathogenicity and infection cycle of Vibrio owensii in larviculture of the ornate spiny lobster (Panulirus ornatus), Applied and Environmental Microbiology, 78, (8) pp. 2841-2849. ISSN 0099-2240 (2012) [Refereed Article]


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Copyright Statement

Copyright 2012 American Society for Microbiology

DOI: doi:10.1128/AEM.07274-11

Abstract

The type strain of Vibrio owensii (DY05) was isolated during an epizootic of aquaculture-reared larvae (phyllosomas) of the ornate spiny lobster (Panulirus ornatus). V. owensii DY05 was formally demonstrated to be the etiological agent of a disease causing rapid and reproducible larval mortality with pathologies similar to those seen during disease epizootics. Vectored challenge via the aquaculture live feed organism Artemia (brine shrimp) caused consistent cumulative mortality rates of 84 to 89% after 72 h, in contrast to variable mortality rates seen after immersion challenge. Histopathological examination of vector-challenged phyllosomas revealed bacterial proliferation in the midgut gland (hepatopancreas) concomitant with epithelial cell necrosis. A fluorescent-protein-labeled V. owensii DY05 transconjugant showed dispersal of single cells in the foregut and hepatopancreas 6 h postexposure, leading to colonization of the entire hepatopancreas within 18 h and eventually systemic infection. V. owensii DY05 is a marine enteropathogen highly virulent to P. ornatus phyllosoma that uses vector-mediated transmission and release from host association to a planktonic existence to perpetuate transfer. This understanding of the infection process will improve targeted biocontrol strategies and enhance the prospects of commercially viable larviculture for this valuable spiny lobster species.

Item Details

Item Type:Refereed Article
Keywords:Panulirus ornatus, phyllosoma, Vibrio owensii, green fluorescent protein, infection cycle
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Crustaceans (excl. Rock Lobster and Prawns)
Author:Goulden, EF (Dr Evan Goulden)
ID Code:94708
Year Published:2012
Web of Science® Times Cited:15
Deposited By:Sustainable Marine Research Collaboration
Deposited On:2014-09-16
Last Modified:2014-10-13
Downloads:211 View Download Statistics

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