2841-2849 Goulden.pdf (2.37 MB)
Pathogenicity and infection cycle of Vibrio owensii in larviculture of the ornate spiny lobster (Panulirus ornatus)
journal contribution
posted on 2023-05-18, 03:20 authored by Evan Goulden, Hall, MR, Bourne, DG, Pereg, LL, Hoj, LThe type strain of Vibrio owensii (DY05) was isolated during an epizootic of aquaculture-reared larvae (phyllosomas) of the ornate spiny lobster (Panulirus ornatus). V. owensii DY05 was formally demonstrated to be the etiological agent of a disease causing rapid and reproducible larval mortality with pathologies similar to those seen during disease epizootics. Vectored challenge via the aquaculture live feed organism Artemia (brine shrimp) caused consistent cumulative mortality rates of 84 to 89% after 72 h, in contrast to variable mortality rates seen after immersion challenge. Histopathological examination of vector-challenged phyllosomas revealed bacterial proliferation in the midgut gland (hepatopancreas) concomitant with epithelial cell necrosis. A fluorescent-protein-labeled V. owensii DY05 transconjugant showed dispersal of single cells in the foregut and hepatopancreas 6 h postexposure, leading to colonization of the entire hepatopancreas within 18 h and eventually systemic infection. V. owensii DY05 is a marine enteropathogen highly virulent to P. ornatus phyllosoma that uses vector-mediated transmission and release from host association to a planktonic existence to perpetuate transfer. This understanding of the infection process will improve targeted biocontrol strategies and enhance the prospects of commercially viable larviculture for this valuable spiny lobster species.
History
Publication title
Applied and Environmental MicrobiologyVolume
78Issue
8Pagination
2841-2849ISSN
0099-2240Department/School
Institute for Marine and Antarctic StudiesPublisher
Amer Soc MicrobiologyPlace of publication
1752 N St Nw, Washington, USA, Dc, 20036-2904Rights statement
Copyright 2012 American Society for MicrobiologyRepository Status
- Open