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Development of a continuous phytoplankton culture system for ocean acidification experiments

Citation

Wynn-Edwards, C and King, R and Kawaguchi, S and Davidson, Andrew and Wright, SW and Nichols, PD and Virtue, P, Development of a continuous phytoplankton culture system for ocean acidification experiments, Water, 6, (6) pp. 1860-1872. ISSN 2073-4441 (2014) [Refereed Article]


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Licensed under Creative Commons Attribution 3.0 Unported (CC BY 3.0) http://creativecommons.org/licenses/by/3.0/

DOI: doi:10.3390/w6061860

Abstract

Around one third of all anthropogenic CO2 emissions have been absorbed by the oceans, causing changes in seawater pH and carbonate chemistry. These changes have the potential to affect phytoplankton, which are critically important for marine food webs and the global carbon cycle. However, our current knowledge of how phytoplankton will respond to these changes is limited to a few laboratory and mesocosm experiments. Long-term experiments are needed to determine the vulnerability of phytoplankton to enhanced pCO2. Maintaining phytoplankton cultures in exponential growth for extended periods of time is logistically difficult and labour intensive. Here we describe a continuous culture system that greatly reduces the time required to maintain phytoplankton cultures, and minimises variation in experimental pCO2 treatments over time. This system is simple, relatively cheap, flexible, and allows long-term experiments to be performed to further our understanding of chronic responses and adaptation by phytoplankton species to future ocean acidification. © 2014 by the authors.

Item Details

Item Type:Refereed Article
Keywords:Phytoplankton Culture System, Ocean Acidification, carbonate chemistry
Research Division:Earth Sciences
Research Group:Oceanography
Research Field:Biological Oceanography
Objective Division:Environment
Objective Group:Other Environment
Objective Field:Antarctic and Sub-Antarctic Oceanography
Author:Wynn-Edwards, C (Dr Cathryn Wynn-Edwards)
Author:Kawaguchi, S (Dr So Kawaguchi)
Author:Davidson, Andrew (Dr Andrew Davidson)
Author:Wright, SW (Dr Simon Wright)
Author:Nichols, PD (Dr Peter Nichols)
Author:Virtue, P (Associate Professor Patti Virtue)
ID Code:94445
Year Published:2014
Web of Science® Times Cited:2
Deposited By:CRC-Antarctic Climate & Ecosystems
Deposited On:2014-09-09
Last Modified:2017-11-01
Downloads:218 View Download Statistics

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