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Development of a continuous phytoplankton culture system for ocean acidification experiments
Citation
Wynn-Edwards, C and King, R and Kawaguchi, S and Davidson, Andrew and Wright, SW and Nichols, PD and Virtue, P, Development of a continuous phytoplankton culture system for ocean acidification experiments, Water, 6, (6) pp. 1860-1872. ISSN 2073-4441 (2014) [Refereed Article]
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Copyright Statement
Licensed under Creative Commons Attribution 3.0 Unported (CC BY 3.0) http://creativecommons.org/licenses/by/3.0/
DOI: doi:10.3390/w6061860
Abstract
Around one third of all anthropogenic CO2 emissions have been absorbed by the oceans, causing changes in seawater pH and carbonate chemistry. These changes have the potential to affect phytoplankton, which are critically important for marine food webs and the global carbon cycle. However, our current knowledge of how phytoplankton will respond to these changes is limited to a few laboratory and mesocosm experiments. Long-term experiments are needed to determine the vulnerability of phytoplankton to enhanced pCO2. Maintaining phytoplankton cultures in exponential growth for extended periods of time is logistically difficult and labour intensive. Here we describe a continuous culture system that greatly reduces the time required to maintain phytoplankton cultures, and minimises variation in experimental pCO2 treatments over time. This system is simple, relatively cheap, flexible, and allows long-term experiments to be performed to further our understanding of chronic responses and adaptation by phytoplankton species to future ocean acidification. © 2014 by the authors.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | Phytoplankton Culture System, Ocean Acidification, carbonate chemistry |
| Research Division: | Earth Sciences |
| Research Group: | Oceanography |
| Research Field: | Biological Oceanography |
| Objective Division: | Environment |
| Objective Group: | Other Environment |
| Objective Field: | Antarctic and Sub-Antarctic Oceanography |
| Author: | Wynn-Edwards, C (Dr Cathryn Wynn-Edwards) |
| Author: | Kawaguchi, S (Dr So Kawaguchi) |
| Author: | Davidson, Andrew (Dr Andrew Davidson) |
| Author: | Wright, SW (Dr Simon Wright) |
| Author: | Nichols, PD (Dr Peter Nichols) |
| Author: | Virtue, P (Associate Professor Patti Virtue) |
| ID Code: | 94445 |
| Year Published: | 2014 |
| Web of Science® Times Cited: | 2 |
| Deposited By: | CRC-Antarctic Climate & Ecosystems |
| Deposited On: | 2014-09-09 |
| Last Modified: | 2017-11-01 |
| Downloads: | 249 View Download Statistics |
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