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Multiplex RT-PCR detection of three common viruses infecting orchids

Citation

Ali, RN and Dann, AL and Cross, PA and Wilson, CR, Multiplex RT-PCR detection of three common viruses infecting orchids, Archives of Virology, 159, (11) pp. 3095-3099. ISSN 0304-8608 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Springer

DOI: doi:10.1007/s00705-014-2161-9

Abstract

A multiplex reverse transcription polymerase chain reaction (RT-PCR) assay was developed for simultaneous detection of three orchid viruses: cymbidium mosaic virus (CymMV), odontoglossum ringspot virus (ORSV), and orchid fleck virus (OFV). Primers were used to amplify nucleocapsid protein gene fragments of 845 bp (ORSV), 505 bp (CymMV) and 160 bp (OFV). A 60-bp amplicon of plant glyceraldehyde-3-phophate dehydrogenase mRNA was included as an internal control against false negatives. The assay was validated against 31 collected plants from six orchid genera and compared with results obtained by transmission electron microscopy (TEM). The RT-PCR assay proved more sensitive than TEM for detection of OFV.

Item Details

Item Type:Refereed Article
Keywords:PCR, diagnostics, orchid fleck virus, cymbidium mosaic virus, odontoglossum ringspot virus
Research Division:Agricultural and Veterinary Sciences
Research Group:Horticultural Production
Research Field:Horticultural Crop Protection (Pests, Diseases and Weeds)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural Crops
Objective Field:Horticultural Crops not elsewhere classified
Author:Ali, RN (Mr Raymond Ali)
Author:Wilson, CR (Associate Professor Calum Wilson)
ID Code:93956
Year Published:2014
Web of Science® Times Cited:5
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2014-08-25
Last Modified:2017-11-06
Downloads:0

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