University of Tasmania
Browse

File(s) under permanent embargo

Multiplex RT-PCR detection of three common viruses infecting orchids

journal contribution
posted on 2023-05-18, 02:50 authored by Ali, R, Dann, AL, Cross, PA, Calum WilsonCalum Wilson
A multiplex reverse transcription polymerase chain reaction (RT-PCR) assay was developed for simultaneous detection of three orchid viruses: cymbidium mosaic virus (CymMV), odontoglossum ringspot virus (ORSV), and orchid fleck virus (OFV). Primers were used to amplify nucleocapsid protein gene fragments of 845 bp (ORSV), 505 bp (CymMV) and 160 bp (OFV). A 60-bp amplicon of plant glyceraldehyde-3-phophate dehydrogenase mRNA was included as an internal control against false negatives. The assay was validated against 31 collected plants from six orchid genera and compared with results obtained by transmission electron microscopy (TEM). The RT-PCR assay proved more sensitive than TEM for detection of OFV.

History

Publication title

Archives of Virology

Volume

159

Issue

11

Pagination

3095-3099

ISSN

0304-8608

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

Springer-Verlag Wien

Place of publication

Sachsenplatz 4-6, Po Box 89, Vienna, Austria, A-1201

Rights statement

Copyright 2014 Springer

Repository Status

  • Restricted

Socio-economic Objectives

Horticultural crops not elsewhere classified

Usage metrics

    University Of Tasmania

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC