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Multiplex RT-PCR detection of three common viruses infecting orchids
journal contribution
posted on 2023-05-18, 02:50 authored by Ali, R, Dann, AL, Cross, PA, Calum WilsonCalum WilsonA multiplex reverse transcription polymerase chain reaction (RT-PCR) assay was developed for simultaneous detection of three orchid viruses: cymbidium mosaic virus (CymMV), odontoglossum ringspot virus (ORSV), and orchid fleck virus (OFV). Primers were used to amplify nucleocapsid protein gene fragments of 845 bp (ORSV), 505 bp (CymMV) and 160 bp (OFV). A 60-bp amplicon of plant glyceraldehyde-3-phophate dehydrogenase mRNA was included as an internal control against false negatives. The assay was validated against 31 collected plants from six orchid genera and compared with results obtained by transmission electron microscopy (TEM). The RT-PCR assay proved more sensitive than TEM for detection of OFV.
History
Publication title
Archives of VirologyVolume
159Issue
11Pagination
3095-3099ISSN
0304-8608Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
Springer-Verlag WienPlace of publication
Sachsenplatz 4-6, Po Box 89, Vienna, Austria, A-1201Rights statement
Copyright 2014 SpringerRepository Status
- Restricted