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Linking oxygen availability with membrane potential maintenance and K+ retention of barley roots: implications for waterlogging stress tolerance

Citation

Zeng, F and Konnerup, D and Shabala, L and Zhou, M and Colmer, TD and Zhang, G and Shabala, S, Linking oxygen availability with membrane potential maintenance and K+ retention of barley roots: implications for waterlogging stress tolerance, Plant, Cell and Environment, 37, (10) pp. 2325-2338. ISSN 0140-7791 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 John Wiley & Sons Ltd.

DOI: doi:10.1111/pce.12422

Abstract

Oxygen deprivation is a key determinant of root growth and functioning under waterlogging. In this work, changes in net K+ flux and membrane potential (MP) of root cells were measured from elongation and mature zones of two barley varieties under hypoxia and anoxia conditions in the medium, and as influenced by ability to transport O2 from the shoot. We show that O2 deprivation results in an immediate K+ loss from roots, in a tissue- and time-specific manner, affecting root K+ homeostasis. Both anoxia and hypoxia induced transient membrane depolarization; the extent of this depolarization varied depending on severity of O2 stress and was less pronounced in a waterlogging-tolerant variety. Intact roots of barley were capable of maintaining H+-pumping activity under hypoxic conditions while disrupting O2 transport from shoot to root resulted in more pronounced membrane depolarization under O2-limited conditions and in anoxia a rapid loss of the cell viability. It is concluded that the ability of root cells to maintain MP and cytosolic K+ homeostasis is central to plant performance under waterlogging, and efficient O2 transport from the shoot may enable operation of the plasma membrane H+-ATPase in roots even under conditions of severe O2 limitation in the soil solution.

Item Details

Item Type:Refereed Article
Keywords:barley, waterlogging, Hordeum vulgare, anoxia, H+-ATPase, hypoxia, internal O2 transport, ion transport, K+ flux
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Improvement (Selection and Breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Barley
Author:Zeng, F (Dr Fanrong Zeng)
Author:Shabala, L (Dr Lana Shabala)
Author:Zhou, M (Associate Professor Meixue Zhou)
Author:Shabala, S (Professor Sergey Shabala)
ID Code:93933
Year Published:2014
Funding Support:Australian Research Council (LP120200516)
Web of Science® Times Cited:12
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2014-08-22
Last Modified:2017-11-06
Downloads:0

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