eCite Digital Repository

Rapid and sensitive analysis of vincristine in human plasma using on-line extraction combined with liquid chromatography/tandem mass spectrometry

Citation

Corona, G and Casetta, B and Sandron, S and Vaccher, E and Toffoli, G, Rapid and sensitive analysis of vincristine in human plasma using on-line extraction combined with liquid chromatography/tandem mass spectrometry, Rapid Communications in Mass Spectrometry, 22, (4) pp. 519-525. ISSN 0951-4198 (2008) [Refereed Article]

Copyright Statement

Copyright 2008 John Wiley & Sons, Ltd.

DOI: doi:10.1002/rcm.3390

Abstract

A simple and rapid method has been developed and validated for the quantitation of vincristine in human plasma by liquid chromatography/tandem mass spectrometry (LC/MS/MS) with atmospheric pressure chemical ionization using on-line solid-phase extraction. The method uses vinblastine as internal standard and the sample preparation is limited just to a plasma protein precipitation step. Further sample clean-up is carried out on-line through a perfusion column preceding an analytical phenyl LC column, the latter directly connected to the mass spectrometer. Quantitation is performed in multiple reaction monitoring mode using the transitions of m/z 825.3  → 765.3 and 811.3 → 751.3 for vincristine and vinblastine respectively. The assay was linear (r2 ≥0.99) in a concentration range from 0.1 to 500 ng/mL. Carry-over, measured on the experimental set-up, was less than 0.04%. Recovery for vincristine and the internal standard was within 9095%. The intra-day and inter-day assay precision ranged from 1.2% to 6.8% RSD while mean percentage deviation from nominal value ranged from 0.01% to 6.1%. The proposed assay was found suitable for pharmacokinetics investigations and clinical therapeutic drug monitoring especially in pediatric cancer patients.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Cancer and Related Disorders
Author:Sandron, S (Dr Sara Sandron)
ID Code:93932
Year Published:2008
Web of Science® Times Cited:23
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2014-08-22
Last Modified:2014-08-27
Downloads:0

Repository Staff Only: item control page