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Rapid and sensitive analysis of vincristine in human plasma using on-line extraction combined with liquid chromatography/tandem mass spectrometry
Citation
Corona, G and Casetta, B and Sandron, S and Vaccher, E and Toffoli, G, Rapid and sensitive analysis of vincristine in human plasma using on-line extraction combined with liquid chromatography/tandem mass spectrometry, Rapid Communications in Mass Spectrometry, 22, (4) pp. 519-525. ISSN 0951-4198 (2008) [Refereed Article]
Copyright Statement
Copyright 2008 John Wiley & Sons, Ltd.
DOI: doi:10.1002/rcm.3390
Abstract
A simple and rapid method has been developed and validated for the quantitation of vincristine in human plasma by liquid chromatography/tandem mass spectrometry (LC/MS/MS) with atmospheric pressure chemical ionization using on-line solid-phase extraction. The method uses vinblastine as internal standard and the sample preparation is limited just to a plasma protein precipitation step. Further sample clean-up is carried out on-line through a perfusion column preceding an analytical phenyl LC column, the latter directly connected to the mass spectrometer. Quantitation is performed in multiple reaction monitoring mode using the transitions of m/z 825.3 → 765.3 and 811.3 → 751.3 for vincristine and vinblastine respectively. The assay was linear (r2 ≥0.99) in a concentration range from 0.1 to 500 ng/mL. Carry-over, measured on the experimental set-up, was less than 0.04%. Recovery for vincristine and the internal standard was within 90–95%. The intra-day and inter-day assay precision ranged from 1.2% to 6.8% RSD while mean percentage deviation from nominal value ranged from 0.01% to 6.1%. The proposed assay was found suitable for pharmacokinetics investigations and clinical therapeutic drug monitoring especially in pediatric cancer patients.
Item Details
Item Type: | Refereed Article |
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Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Health |
Objective Group: | Clinical health |
Objective Field: | Clinical health not elsewhere classified |
UTAS Author: | Sandron, S (Dr Sara Sandron) |
ID Code: | 93932 |
Year Published: | 2008 |
Web of Science® Times Cited: | 32 |
Deposited By: | Austn Centre for Research in Separation Science |
Deposited On: | 2014-08-22 |
Last Modified: | 2014-08-27 |
Downloads: | 0 |
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