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Detection of chronic wasting disease in the lymph nodes of free-ranging cervids by real-time quaking-induced conversion

Citation

Haley, NJ and Carver, S and Hoon-Hanks, LL and Henderson, DM and Davenport, KA and Bunting, E and Gray, S and Trindle, B and Galeota, J and LeVan, I and Dubovos, T and Shelton, P and Hoover, EA, Detection of chronic wasting disease in the lymph nodes of free-ranging cervids by real-time quaking-induced conversion, Journal of Clinical Microbiology, 52, (9) pp. 3237-3243. ISSN 0095-1137 (2014) [Refereed Article]


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Coopyright 2014 American Society for Microbiology

DOI: doi:10.1128/JCM.01258-14

Abstract

Chronic wasting disease (CWD), a transmissible spongiform encephalopathy of deer, elk, and moose, is the only prion disease affecting free-ranging animals. Since the disease was first identified in northern Colorado and southern Wyoming in 1967, new epidemic foci of the disease have been identified in 20 additional states, as well as two Canadian provinces and the Republic of South Korea. Identification of CWD-affected animals currently requires postmortem analysis of brain or lymphoid tissues using immunohistochemistry (IHC) or an enzyme-linked immunosorbent assay (ELISA), with no practical way to evaluate potential strain types or to investigate the epidemiology of existing or novel foci of disease. Using a standardized real-time (RT)-quaking-induced conversion (QuIC) assay, a seeded amplification assay employing recombinant prion protein as a conversion substrate and thioflavin T (ThT) as an amyloid-binding fluorophore, we analyzed, in a blinded manner, 1,243 retropharyngeal lymph node samples from white-tailed deer, mule deer, and moose, collected in the field from areas with current or historic CWD endemicity. RT-QuIC results were then compared with those obtained by conventional IHC and ELISA, and amplification metrics using ThT and thioflavin S were examined in relation to the clinical history of the sampled deer. The results indicate that RT-QuIC is useful for both identifying CWD-infected animals and facilitating epidemiological studies in areas in which CWD is endemic or not endemic.

Item Details

Item Type:Refereed Article
Keywords:chronic wasting disease, cervid, prion, epidemiology, disease ecology, deer
Research Division:Biological Sciences
Research Group:Evolutionary Biology
Research Field:Host-Parasite Interactions
Objective Division:Health
Objective Group:Public Health (excl. Specific Population Health)
Objective Field:Disease Distribution and Transmission (incl. Surveillance and Response)
Author:Carver, S (Dr Scott Carver)
ID Code:93924
Year Published:2014
Web of Science® Times Cited:9
Deposited By:Zoology
Deposited On:2014-08-22
Last Modified:2017-11-06
Downloads:195 View Download Statistics

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