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Evidence that indole-3-acetic acid is not synthesized via the indole-3-acetamide pathway in pea roots

Citation

Quittenden, LJ and McAdam, EL and Davies, NW and Ross, JJ, Evidence that indole-3-acetic acid is not synthesized via the indole-3-acetamide pathway in pea roots, Journal of Plant Growth Regulation, 33, (4) pp. 831-836. ISSN 0721-7595 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Springer

DOI: doi:10.1007/s00344-014-9431-3

Abstract

The biosynthetic route of the key plant hormone, indole-3-acetic acid (IAA) has confounded generations of biologists. Evidence in higher plants has implicated two auxin intermediates with roles established in bacteria: indole-3-acetamide (IAM) and indole-3-pyruvic acid. Herein, the IAM pathway is investigated in pea (Pisum sativum), a model legume. The compound was not detected in pea tissue, although evidence was obtained for its presence in Arabidopsis, tobacco, and maize. Deuterium-labeled tryptophan was not converted to IAM in pea roots, despite being converted to IAA. After feeds of deuterium-labeled IAM, label was recovered in the IAA conjugate IAA-aspartate (IAAsp), although there was little or no labeling of IAA itself. Plants treated with IAM did not exhibit high-IAA phenotypes, and did not accumulate IAA. This evidence, taken together, indicates that although exogenous IAM may be converted to IAA (and further to IAAsp), the IAM pathway does not operate naturally in pea roots.

Item Details

Item Type:Refereed Article
Keywords:indole-3-acetic acid, biosynthesis, auxin, indole-3-acetamide, pea, Pisum sativum
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Quittenden, LJ (Dr Laura Quittenden)
Author:McAdam, EL (Dr Erin McAdam)
Author:Davies, NW (Associate Professor Noel Davies)
Author:Ross, JJ (Associate Professor John Ross)
ID Code:93816
Year Published:2014
Web of Science® Times Cited:1
Deposited By:Plant Science
Deposited On:2014-08-19
Last Modified:2017-11-02
Downloads:0

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