eCite Digital Repository

Normal-phase high-performance counter-current chromatography for the fractionation of dissolved organic matter from a freshwater source

Citation

Sandron, S and Nesterenko, PN and McCaul, MV and Kelleher, B and Paull, B, Normal-phase high-performance counter-current chromatography for the fractionation of dissolved organic matter from a freshwater source, Journal of Separation Science, 37, (1-2) pp. 135-142. ISSN 1615-9306 (2014) [Refereed Article]

Copyright Statement

Copyright 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

DOI: doi:10.1002/jssc.201300634

Abstract

Normal-phase high-performance counter-current chromatography (HPCCC) is used to obtain a preliminary fractionation of components in dissolved organic matter (DOM) from a freshwater source. The HPCCC solvent system involved a normal-phase approach with water/methanol (1:1) as the lower stationary phase and hexane/ethyl acetate (1:1) as the upper mobile phase. The critical experiment parameters were optimised: revolution speed 1800 rpm and flow rate 0.15 mL/min. Under these conditions 50 μL of a 0.50 mg/mL DOM solution was loaded. The detection wavelength was monitored at 330 nm in order to isolate the main portion of DOM, which includes substances such as carboxyl-rich alicyclic molecules. By optimising this system it was possible to isolate materials that, according to GC–MS, can be related to molecules with an analogous structural background. Where fraction analysis was not suitable for GC–MS, RP-HPLC with UV absorbance detection was used, showing unique chromatograms for each fraction at both 210 and 330 nm.

Item Details

Item Type:Refereed Article
Keywords:counter-current chromatography, dissolved organic matter, freshwater source, sample fractionation
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Sandron, S (Dr Sara Sandron)
Author:Nesterenko, PN (Professor Pavel Nesterenko)
Author:Paull, B (Professor Brett Paull)
ID Code:93677
Year Published:2014
Web of Science® Times Cited:3
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2014-08-12
Last Modified:2017-10-27
Downloads:0

Repository Staff Only: item control page