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Normal-phase high-performance counter-current chromatography for the fractionation of dissolved organic matter from a freshwater source
journal contribution
posted on 2023-05-18, 02:33 authored by Sandron, S, Nesterenko, PN, McCaul, MV, Kelleher, B, Brett PaullBrett PaullNormal-phase high-performance counter-current chromatography (HPCCC) is used to obtain a preliminary fractionation of components in dissolved organic matter (DOM) from a freshwater source. The HPCCC solvent system involved a normal-phase approach with water/methanol (1:1) as the lower stationary phase and hexane/ethyl acetate (1:1) as the upper mobile phase. The critical experiment parameters were optimised: revolution speed 1800 rpm and flow rate 0.15 mL/min. Under these conditions 50 μL of a 0.50 mg/mL DOM solution was loaded. The detection wavelength was monitored at 330 nm in order to isolate the main portion of DOM, which includes substances such as carboxyl-rich alicyclic molecules. By optimising this system it was possible to isolate materials that, according to GC–MS, can be related to molecules with an analogous structural background. Where fraction analysis was not suitable for GC–MS, RP-HPLC with UV absorbance detection was used, showing unique chromatograms for each fraction at both 210 and 330 nm.
History
Publication title
Journal of Separation ScienceVolume
37Issue
1-2Pagination
135-142ISSN
1615-9306Department/School
School of Natural SciencesPublisher
Wiley - V C H Verlag GmbH & CoPlace of publication
GermanyRights statement
Copyright 2013 WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimRepository Status
- Restricted