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Normal-phase high-performance counter-current chromatography for the fractionation of dissolved organic matter from a freshwater source

journal contribution
posted on 2023-05-18, 02:33 authored by Sandron, S, Nesterenko, PN, McCaul, MV, Kelleher, B, Brett PaullBrett Paull
Normal-phase high-performance counter-current chromatography (HPCCC) is used to obtain a preliminary fractionation of components in dissolved organic matter (DOM) from a freshwater source. The HPCCC solvent system involved a normal-phase approach with water/methanol (1:1) as the lower stationary phase and hexane/ethyl acetate (1:1) as the upper mobile phase. The critical experiment parameters were optimised: revolution speed 1800 rpm and flow rate 0.15 mL/min. Under these conditions 50 μL of a 0.50 mg/mL DOM solution was loaded. The detection wavelength was monitored at 330 nm in order to isolate the main portion of DOM, which includes substances such as carboxyl-rich alicyclic molecules. By optimising this system it was possible to isolate materials that, according to GC–MS, can be related to molecules with an analogous structural background. Where fraction analysis was not suitable for GC–MS, RP-HPLC with UV absorbance detection was used, showing unique chromatograms for each fraction at both 210 and 330 nm.

History

Publication title

Journal of Separation Science

Volume

37

Issue

1-2

Pagination

135-142

ISSN

1615-9306

Department/School

School of Natural Sciences

Publisher

Wiley - V C H Verlag GmbH & Co

Place of publication

Germany

Rights statement

Copyright 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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