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Ion transport in broad bean leaf mesophyll under saline conditions


Percey, WJ and Shabala, L and Breadmore, MC and Guijt, RM and Bose, J and Shabala, S, Ion transport in broad bean leaf mesophyll under saline conditions, Planta, 240, (4) pp. 729-743. ISSN 0032-0935 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Springer-Verlag Berlin Heidelberg

DOI: doi:10.1007/s00425-014-2117-z


Modulation in ion transport of broad bean (Vicia faba L.) mesophyll to light under increased apoplastic salinity stress was investigated using vibrating ion-selective microelectrodes (the MIFE technique). Increased apoplastic Na+ significantly affected mesophyll cells ability to respond to light by modulating ion transport across their membranes. Elevated apoplastic Na+ also induced a significant K+ efflux from mesophyll tissue. This efflux was mediated predominately by potassium outward rectifying channels (84 %) and the remainder of the efflux was through non-selective cation channels. NaCl treatment resulted in a reduction in photosystem II efficiency in a dose- and time-dependent manner. In particular, reductions in Fv′/Fm′ were linked to K+ homeostasis in the mesophyll tissue. Increased apoplastic Na+ concentrations induced vanadate-sensitive net H+ efflux, presumably mediated by the plasma membrane H+-ATPase. It is concluded that the observed pumpís activation is essential for the maintenance of membrane potential and ion homeostasis in the cytoplasm of mesophyll under salt stress.

Item Details

Item Type:Refereed Article
Keywords:salinity, broad bean, mesophyll, flux, membrane potential, potassium, photosynthesis, H+-ATPase
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Percey, WJ (Mr William Percey)
UTAS Author:Shabala, L (Associate Professor Lana Shabala)
UTAS Author:Breadmore, MC (Professor Michael Breadmore)
UTAS Author:Guijt, RM (Dr Rosanne Guijt)
UTAS Author:Bose, J (Dr Jayakumar Bose)
UTAS Author:Shabala, S (Professor Sergey Shabala)
ID Code:93364
Year Published:2014
Web of Science® Times Cited:17
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2014-07-29
Last Modified:2017-11-02

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