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Ion transport in broad bean leaf mesophyll under saline conditions
journal contribution
posted on 2023-05-18, 02:18 authored by Percey, WJ, Svetlana ShabalaSvetlana Shabala, Michael BreadmoreMichael Breadmore, Rosanne Guijt, Bose, J, Sergey ShabalaSergey ShabalaModulation in ion transport of broad bean (Vicia faba L.) mesophyll to light under increased apoplastic salinity stress was investigated using vibrating ion-selective microelectrodes (the MIFE technique). Increased apoplastic Na+ significantly affected mesophyll cells ability to respond to light by modulating ion transport across their membranes. Elevated apoplastic Na+ also induced a significant K+ efflux from mesophyll tissue. This efflux was mediated predominately by potassium outward rectifying channels (84 %) and the remainder of the efflux was through non-selective cation channels. NaCl treatment resulted in a reduction in photosystem II efficiency in a dose- and time-dependent manner. In particular, reductions in Fv′/Fm′ were linked to K+ homeostasis in the mesophyll tissue. Increased apoplastic Na+ concentrations induced vanadate-sensitive net H+ efflux, presumably mediated by the plasma membrane H+-ATPase. It is concluded that the observed pump’s activation is essential for the maintenance of membrane potential and ion homeostasis in the cytoplasm of mesophyll under salt stress.
History
Publication title
PlantaVolume
240Issue
4Pagination
729-743ISSN
0032-0935Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
Springer-VerlagPlace of publication
175 Fifth Ave, New York, USA, Ny, 10010Rights statement
Copyright 2014 Springer-Verlag Berlin HeidelbergRepository Status
- Restricted