Recommendations were developed to help aquatic scientists select which curve fitting method should be used to fit data that are expected to follow rectangular hyperbolic relationships. Rectangular hyperbolae of the form V = (V_maxS)/(K_m + S), where V is a biological rate and S is the concentration of some substrate, are widely used by researchers to model the kinetics of processes such as enzyme activity versus substrate concentration, nutrient uptake versus nutrient concentration, and grazing and growth rate versus prey concentration. A variety of procedures exist to estimate the parameters V_max (the rate of the process at saturating substrate concentration) and K_m, (the concentration of S at which half the saturated rate is achieved). There has been extensive discussion in the biochemical and ecological literature as to which fitting method is most appropriate, based largely on theoretical and statistical considerations. However, the assumptions inherent in these fitting procedures are typically violated by the data obtained in many field and laboratory studies, e.g. the measurement of S has an associated error, or error levels in the measurement of V may not be constant across S. Thus, there is a problem predicting a priori which fitting method should be used. In this study, this problem was approached using Monte Carlo simulations. Data sets with known V_{max and Km were constructed for 5 different data cases, ranging from data sets where saturation was not achieved to data sets where very few sub-saturated measurements were available. Random, normally distributed errors were assigned to each point based on a 10 %, 20 % or 50 % constant or variable error in the estimate of V, or 20 % error in both S and V Six fitting procedures were applied including linear methods (Lineweaver-Burk; Eadie-Hofstee; Hanes-Woolf), the median method (Eisenthal and Cornish-Bowden), and non-linear least-squares methods (Cleland-Wilkinson; Tseng-Hsu). Non-linear methods were generally superior, but for data sets with low error (10%) all methods gave almost equally accurate results. Data with constant error were more difficult to fit than those where error varied with V. Criteria for selecting a fitting method based on data characteristics are discussed and applied to actual data sets}

Item Type:	Refereed Article
Keywords:	modelling physiological responses, seaweed, invertebrates, microalgae
Research Division:	Biological Sciences
Research Group:	Ecology
Research Field:	Ecological Physiology
Objective Division:	Expanding Knowledge
Objective Group:	Expanding Knowledge
Objective Field:	Expanding Knowledge in the Biological Sciences
UTAS Author:	Hurd, CL (Professor Catriona Hurd)
ID Code:	93177
Year Published:	1994
Deposited By:	IMAS Research and Education Centre
Deposited On:	2014-07-15
Last Modified:	2014-08-01
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