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Annexin 1 regulates the H2O2-induced calcium signature in Arabidopsis thaliana roots
Citation
Richards, SL and Laohavisit, A and Mortimer, JC and Shabala, L and Swarbreck, SM and Shabala, S and Davies, JM, Annexin 1 regulates the H2O2-induced calcium signature in Arabidopsis thaliana roots, The Plant Journal, 77, (1) pp. 136-145. ISSN 0960-7412 (2014) [Refereed Article]
Copyright Statement
Copyright 2013 The Authors
Abstract
Hydrogen peroxide is the most stable of the reactive oxygen species (ROS) and is a regulator of development, immunity and adaptation to stress. It frequently acts by elevating cytosolic free Ca2+ ([Ca2+]cyt) as a second messenger, with activation of plasma membrane Ca2+-permeable influx channels as a fundamental part of this process. At the genetic level, to date only the Ca2+-permeable Stelar K+ Outward Rectifier (SKOR) channel has been identified as being responsive to hydrogen peroxide. We show here that the ROS-regulated Ca2+ transport protein Annexin 1 in Arabidopsis thaliana (AtANN1) is involved in regulating the root epidermal [Ca2+]cyt response to stress levels of extracellular hydrogen peroxide. Peroxide-stimulated [Ca2+]cyt elevation (determined using aequorin luminometry) was aberrant in roots and root epidermal protoplasts of the Atann1 knockout mutant. Similarly, peroxide-stimulated net Ca2+ influx and K+ efflux were aberrant in Atann1 root mature epidermis, determined using extracellular vibrating ion-selective microelectrodes. Peroxide induction of GSTU1 (Glutathione-S-Transferase1 Tau 1), which is known to be [Ca2+]cyt-dependent was impaired in mutant roots, consistent with a lesion in signalling. Expression of AtANN1 in roots was suppressed by peroxide, consistent with the need to restrict further Ca2+ influx. Differential regulation of annexin expression was evident, with AtANN2 down-regulation but up-regulation of AtANN3 and AtANN4. Overall the results point to involvement of AtANN1 in shaping the root peroxide-induced [Ca2+]cyt signature and downstream signalling.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | annexin, ROS, oxidative stress, peroxide, calcium, root, glutathione, Arabidopsis |
| Research Division: | Biological Sciences |
| Research Group: | Plant Biology |
| Research Field: | Plant Physiology |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding Knowledge |
| Objective Field: | Expanding Knowledge in the Biological Sciences |
| Author: | Shabala, L (Dr Lana Shabala) |
| Author: | Shabala, S (Professor Sergey Shabala) |
| ID Code: | 93067 |
| Year Published: | 2014 |
| Web of Science® Times Cited: | 32 |
| Deposited By: | Tasmanian Institute of Agriculture |
| Deposited On: | 2014-07-09 |
| Last Modified: | 2017-11-02 |
| Downloads: | 0 |
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