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Cyclic mononucleotides modulate potassium and calcium flux responses to H2O2 in Arabidopsis roots

Citation

Ordonez, NM and Marondedze, C and Thomas, L and Pasqualini, S and Shabala, L and Shabala, S and Gehring, C, Cyclic mononucleotides modulate potassium and calcium flux responses to H2O2 in Arabidopsis roots, FEBS Letters, 588, (6) pp. 1008-1015. ISSN 0014-5793 (2014) [Refereed Article]


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Licensed under Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0) http://creativecommons.org/licenses/by-nc-nd/3.0/

DOI: doi:10.1016/j.febslet.2014.01.062

Abstract

Cyclic mononucleotides are messengers in plant stress responses. Here we show that hydrogen peroxide (H2O2) induces rapid net K+-efflux and Ca2+-influx in Arabidopsis roots. Pre-treatment with either 10 μM cAMP or cGMP for 1 or 24 h does significantly reduce net K+-leakage and Ca2+-influx, and in the case of the K+-fluxes, the cell permeant cyclic mononucleotides are more effective. We also examined the effect of 10 μM of the cell permeant 8-Br-cGMP on the Arabidopsis microsomal proteome and noted a specific increase in proteins with a role in stress responses and ion transport, suggesting that cGMP is sufficient to directly and/or indirectly induce complex adaptive changes to cellular stresses induced by H2O2.

Item Details

Item Type:Refereed Article
Keywords:oxidative stress, potassium, calcium, plant stress, hydrogen peroxide, cyclic mononucleotide, cAMP, cGMP, ion flux, proteomic, Arabidopsis thaliana
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Other Plant Production and Plant Primary Products
Objective Field:Plant Production and Plant Primary Products not elsewhere classified
Author:Shabala, L (Dr Lana Shabala)
Author:Shabala, S (Professor Sergey Shabala)
ID Code:93059
Year Published:2014
Web of Science® Times Cited:18
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2014-07-09
Last Modified:2017-11-02
Downloads:228 View Download Statistics

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