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A comparison of potato seed-tuber sampling strategies using visual and DNA analyses to estimate incidence of major seed tuber-borne pathogens


Tegg, RS and Corkrey, R and Wilson, CR, A comparison of potato seed-tuber sampling strategies using visual and DNA analyses to estimate incidence of major seed tuber-borne pathogens, European Journal of Plant Pathology, 139, (2) pp. 359-367. ISSN 0929-1873 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Springer

DOI: doi:10.1007/s10658-014-0392-y


Potato seed certification is a disease management tool that minimises the risk of spreading seed tuber-borne inoculum of infectious diseases. Traditionally, certification sampling strategies have relied upon visual assessment of a seedlot from samples taken at one or two points within the load of seed tubers. However methodologies in selection of tuber samples have not been critically assessed for their precision in estimating disease load. This study presents an analysis of 37 potato seedlots over a 3 year period. Analysis of sample data using receiver operating curves (ROCs) indicates that point sampling taking two samples of 100 tubers at the beginning and end of a seedlot gives equivalent disease estimation as a continuous sampling strategy taking ten samples of 20 tubers randomly throughout the seedlot, although at lower statistical precision. This was confirmed both by visual assessment of tuber-borne disease and by analysis of pathogen DNA content from tuber peel. Across the 3 years of study, powdery scab and black scurf were themajor seed tuberborne diseases recognised and this corresponded with high levels of pathogen DNA from peel analysis for both Spongospora subterranea and Rhizoctonia solani AG3 respectively.

Item Details

Item Type:Refereed Article
Keywords:potato tuber seed pathogens, certification, DNA quantification, seed tuber certification, powdery scab, black scurf, root knot nematode, common scab, receiver operating curves
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Horticultural production
Research Field:Horticultural crop protection (incl. pests, diseases and weeds)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural crops
Objective Field:Field grown vegetable crops
UTAS Author:Tegg, RS (Dr Robert Tegg)
UTAS Author:Corkrey, R (Dr Ross Corkrey)
UTAS Author:Wilson, CR (Professor Calum Wilson)
ID Code:92766
Year Published:2014
Web of Science® Times Cited:14
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2014-06-26
Last Modified:2017-11-06

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