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A comparison of potato seed-tuber sampling strategies using visual and DNA analyses to estimate incidence of major seed tuber-borne pathogens
Citation
Tegg, RS and Corkrey, R and Wilson, CR, A comparison of potato seed-tuber sampling strategies using visual and DNA analyses to estimate incidence of major seed tuber-borne pathogens, European Journal of Plant Pathology, 139, (2) pp. 359-367. ISSN 0929-1873 (2014) [Refereed Article]
Copyright Statement
Copyright 2014 Springer
DOI: doi:10.1007/s10658-014-0392-y
Abstract
Potato seed certification is a disease management
tool that minimises the risk of spreading seed
tuber-borne inoculum of infectious diseases.
Traditionally, certification sampling strategies have relied
upon visual assessment of a seedlot from samples
taken at one or two points within the load of seed tubers.
However methodologies in selection of tuber samples
have not been critically assessed for their precision in
estimating disease load. This study presents an analysis
of 37 potato seedlots over a 3 year period. Analysis of
sample data using receiver operating curves (ROCs)
indicates that point sampling taking two samples of
100 tubers at the beginning and end of a seedlot gives
equivalent disease estimation as a continuous sampling
strategy taking ten samples of 20 tubers randomly
throughout the seedlot, although at lower statistical precision.
This was confirmed both by visual assessment of
tuber-borne disease and by analysis of pathogen DNA
content from tuber peel. Across the 3 years of study,
powdery scab and black scurf were themajor seed tuberborne
diseases recognised and this corresponded with
high levels of pathogen DNA from peel analysis for
both Spongospora subterranea and Rhizoctonia solani
AG3 respectively.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | potato tuber seed pathogens, certification, DNA quantification, seed tuber certification, powdery scab, black scurf, root knot nematode, common scab, receiver operating curves |
| Research Division: | Agricultural and Veterinary Sciences |
| Research Group: | Horticultural Production |
| Research Field: | Horticultural Crop Protection (Pests, Diseases and Weeds) |
| Objective Division: | Plant Production and Plant Primary Products |
| Objective Group: | Horticultural Crops |
| Objective Field: | Vegetables |
| Author: | Tegg, RS (Dr Robert Tegg) |
| Author: | Corkrey, R (Dr Ross Corkrey) |
| Author: | Wilson, CR (Associate Professor Calum Wilson) |
| ID Code: | 92766 |
| Year Published: | 2014 |
| Web of Science® Times Cited: | 13 |
| Deposited By: | Tasmanian Institute of Agriculture |
| Deposited On: | 2014-06-26 |
| Last Modified: | 2017-11-06 |
| Downloads: | 0 |
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