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Bovine-specific nucleotide polymorphisms and mRNA expression of the growth hormone secretagogue receptor 1a (GHSR1a) gene and its genetic association with growth and carcass traits
Citation
Komatsu, M and Sato, Y and Fujimori, Y and Itoh, T and Satoh, M and Nishio, M and Sasaki, O and Takahashi, H and Malau-Aduli, AEO, Bovine-specific nucleotide polymorphisms and mRNA expression of the growth hormone secretagogue receptor 1a (GHSR1a) gene and its genetic association with growth and carcass traits, Journal of Molecular and Genetic Medicine, 8, (2) Article 110. ISSN 1747-0862 (2014) [Refereed Article]
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Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) http://creativecommons.org/licenses/by/4.0/
Official URL: http://www.omicsonline.com/open-access/molecular-g...
DOI: doi:10.4172/1747-0862.1000110
Abstract
The growth hormone secretagogue receptor 1a (GHSR1a) is involved in many important
functions including growth hormone (GH) secretion and appetite regulation and other
important functions. We reveal herein, the unravelling of bovine-specific 5’untranslated
region (5’UTR) microsatellite polymorphisms, a 3bp-indel in exon 1 (DelR2402) and two
different kinds of transcripts of the GHSR1a gene (spliced, without a microsatellite with in
the 5’UTR (GHSR1a); and non-spliced, with the microsatellite (GHSR1b)). A total of 17
alleles ((TG)10~33) in the 5’UTR microsatellite was found in 11 cattle breeds. Furthermore,
we found the DelR242 (3R) allele, a truncated 3-arginine residue (3R) (major type: 4
arginine residues (4R)) within the intracellular loop 3 of GHSR1a protein in Japanese
Shorthorn with a high frequency of 0.43 compared to the low frequency of 0.00~0.09 in
other cattle breeds. We carried out a genetic association study between the 5’UTR
microsatellite and growth and carcass traits in 1,285 steers. Statistical analysis revealed that
the 5’UTR microsatellite locus had a significant additive effect on carcass weight (CW) and
average daily gain (ADG). The 19-TG allele had a significantly desirable effect on these
traits. We proposed a translational hypothesis that the association is due to differences in
the secondary structure of GHSR1b mRNA among the GHSR1a gene haplotypes. We also
examined age-related changes in the expressions of GHSR1a and GHSR1b in many cattle
tissues. The GHSR1a mRNA expression in the arcuate nucleus of post-weaning calves was
more than 10-fold higher than those of pre-weaning calves and cows. In peripheral tissues,
there were 3 marked differences in mRNA expression between cattle, humans and mice, as
follows: (1) the GHSR1a mRNA expression in the liver is high in cattle and very low in
3
humans and mice; (2) the GHSR1b mRNA expression in the liver is low in cattle and high in humans; (3) the GHSR1b mRNA expression in the pancreas is very high in cattle.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | Ghrelin Receptor (GHSR), microsatellite, DelR242, mRNA expression, mRNA secondary structure, bovine-specific, growth hormone secretagogue receptor 1a (GHSR1a) gene, beef cattle, growth, carcass |
| Research Division: | Agricultural and Veterinary Sciences |
| Research Group: | Animal Production |
| Research Field: | Animal Breeding |
| Objective Division: | Animal Production and Animal Primary Products |
| Objective Group: | Livestock Raising |
| Objective Field: | Beef Cattle |
| Author: | Malau-Aduli, AEO (Associate Professor Aduli Malau-Aduli) |
| ID Code: | 92637 |
| Year Published: | 2014 |
| Deposited By: | Tasmanian Institute of Agriculture |
| Deposited On: | 2014-06-25 |
| Last Modified: | 2015-03-30 |
| Downloads: | 349 View Download Statistics |
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