eCite Digital Repository

Endoderm complexity in the mouse gastrula is revealed through the expression of spink3


Goh, HN and Rathjen, PD and Familari, M and Rathjen, J, Endoderm complexity in the mouse gastrula is revealed through the expression of spink3, BioResearch Open Access, 3, (3) pp. 98-109. ISSN 2164-7844 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Mary Ann Liebert, Inc. This is a copy of an article published in the BioResearch Open Access 2014 BioResearch Open Access is available online at:

DOI: doi:10.1089/biores.2014.0010


Endoderm formation in the mammalian embryo occurs first in the blastocyst, when the primitive endoderm and pluripotent cells resolve into separate lineages, and again during gastrulation, when the definitive endoderm progenitor population emerges from the primitive streak. The formation of the definitive endoderm can be modeled using pluripotent cell differentiation in culture. The differentiation of early primitive ectoderm-like (EPL) cells, a pluripotent cell population formed from embryonic stem (ES) cells, was used to identify and characterize definitive endoderm formation. Expression of serine peptidase inhibitor, Kazal type 3 (Spink3) was detected in EPL cell-derived endoderm, and in a band of endoderm immediately distal to the embryonic-extra-embryonic boundary in pregastrula and gastrulating embryos. Later expression marked a region of endoderm separating the yolk sac from the developing gut. In the embryo, Spink3 expression marked a region of endoderm comprising the distal visceral endoderm, as determined by an endocytosis assay, and the proximal region of the definitive endoderm. This region was distinct from the more distal definitive endoderm population, marked by thyrotropin-releasing hormone (Trh). Endoderm expressing either Spink3 or Trh could be formed during EPL cell differentiation, and the prevalence of these populations could be influenced by culture medium and growth factor addition. Moreover, further differentiation suggested that the potential of these populations differed. These approaches have revealed an unexpected complexity in the definitive endoderm lineage, a complexity that will need to be accommodated in differentiation protocols to ensure the formation of the appropriate definitive endoderm progenitor in the future.

Item Details

Item Type:Refereed Article
Keywords:cellular biology, developmental biology, stem cells
Research Division:Biological Sciences
Research Group:Biochemistry and cell biology
Research Field:Cell development, proliferation and death
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Rathjen, PD (Professor Peter Rathjen)
UTAS Author:Rathjen, J (Dr Joy Rathjen)
ID Code:92612
Year Published:2014
Deposited By:Menzies Institute for Medical Research
Deposited On:2014-06-24
Last Modified:2015-02-06

Repository Staff Only: item control page