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Identification of dendritic cells, B cell and T cell subsets in Tasmanian devil lymphoid tissue; evidence for poor immune cell infiltration into devil facial tumors

Citation

Howson, LJ and Morris, KM and Kobayashi, T and Tovar, C and Kreiss, A and Papenfuss, AT and Corcoran, L and Belov, K and Woods, GM, Identification of dendritic cells, B cell and T cell subsets in Tasmanian devil lymphoid tissue; evidence for poor immune cell infiltration into devil facial tumors, Anatomical Record, 297, (5) pp. 925-938. ISSN 1932-8486 (2014) [Refereed Article]


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Copyright Statement

Copyright 2014 the Authors-This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License,(CC BY-NC-ND 2.0 AU) which permits use and distribution in any medium, provided the origi- nal work is properly cited, the use is non-commercial and no modifications or adaptations are made.

DOI: doi:10.1002/ar.22904

Abstract

The Tasmanian devil is under threat of extinction due to the transmissible devil facial tumor disease (DFTD). This fatal tumor is an allograft that does not induce an immune response, raising questions about the activity of Tasmanian devil immune cells. T and B cell analysis has been limited by a lack of antibodies, hence the need to produce such reagents. Amino acid sequence analysis revealed that CD4, CD8, IgM, and IgG were closely related to other marsupials. Monoclonal antibodies were produced against CD4, CD8, IgM, and IgG by generating bacterial fusion proteins. These, and commercial antibodies against CD1a and CD83, identified T cells, B cells and dendritic cells by immunohistochemistry. CD4+ and CD8+ T cells were identified in pouch young thymus, adult lymph nodes, spleen, bronchus- and gut-associated lymphoid tissue. Their anatomical distribution was characteristic of mammalian lymphoid tissues with more CD4+ than CD8+ cells in lymph nodes and splenic white pulp. IgM+ and IgG+ B cells were identified in adult lymph nodes, spleen, bronchus-associated lymphoid tissue and gut-associated lymphoid tissue, with more IgM+ than IgG+ cells. Dendritic cells were identified in lymph node, spleen and skin. This distribution is consistent with eutherian mammals and other marsupials, indicating they have the immune cell subsets for an anti-tumor immunity. Devil facial tumor disease tumors contained more CD8+ than CD4+ cells, but in low numbers. There were also low numbers of CD1a+ and MHC class II+ cells, but no CD83+ IgM+ or IgG+ B cells, consistent with poor immune cell infiltration. © 2014 The Authors.

Item Details

Item Type:Refereed Article
Keywords:devil facial tumour disease; immunohistochemistry; lymphocyte subsets; dendritic cells; marsupial immunology; monoclonal antibody development
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Veterinary sciences
Research Field:Veterinary immunology
Objective Division:Environmental Management
Objective Group:Terrestrial systems and management
Objective Field:Terrestrial biodiversity
UTAS Author:Howson, LJ (Miss Lauren Howson)
UTAS Author:Kobayashi, T (Mr Takumi Kobayashi)
UTAS Author:Tovar, C (Dr Cesar Tovar Lopez)
UTAS Author:Kreiss, A (Dr Alexandre Kreiss)
UTAS Author:Woods, GM (Professor Gregory Woods)
ID Code:92165
Year Published:2014
Funding Support:Australian Research Council (DP130100715 )
Web of Science® Times Cited:27
Deposited By:Menzies Institute for Medical Research
Deposited On:2014-06-07
Last Modified:2017-11-09
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