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Analysis of the central nervous system transcriptome of the eastern rock lobster Sagmariasus verreauxi reveals Its putative neuropeptidome

Citation

Ventura, T and Cummins, SF and Fitzgibbon, Q and Battaglene, S and Elizur, A, Analysis of the central nervous system transcriptome of the eastern rock lobster Sagmariasus verreauxi reveals Its putative neuropeptidome, PLoS One, 9, (5) Article e97323. ISSN 1932-6203 (2014) [Refereed Article]


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Copyright Statement

Copyright 2014 The Authors-This is an open-access article distributed under the terms of the Creative Commons Attribution License, (CC BY 4.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

DOI: doi:10.1371/journal.pone.0097323

Abstract

Neuropeptides have been discovered in many arthropod species including crustaceans. The nature of their biological function is well studied and varies from behavior modulation to physiological regulation of complex biochemical processes such as metabolism, molt and reproduction. Due to their key role in these fundamental processes, neuropeptides are often targeted for modulating these processes to align with market demands in commercially important species. We generated a comprehensive transcriptome of the eyestalk and brain of one of the few commercially important spiny lobster species in the southern Hemisphere, the Eastern rock lobster Sagmariasus verreauxi and mined it for novel neuropeptide and protein hormone-encoding transcripts. We then characterized the predicted mature hormones to verify their validity based on conserved motifs and features known from previously reported hormones. Overall, 37 transcripts which are predicted to encode mature full-length/partial peptides/proteins were identified, representing 21 peptide/protein families/subfamilies. All transcripts had high similarity to hormones that were previously characterized in other decapod crustacean species or, where absent in crustaceans, in other arthropod species. These included, in addition to other proteins previously described in crustaceans, prohormone-3 and prohormone-4 which were previously identified only in insects. A homolog of the crustacean female sex hormone (CFSH), recently found to be female-specific in brachyuran crabs was found to have the same levels of expression in both male and female eyestalks, suggesting that the CFSH female specificity is not conserved throughout decapod crustaceans. Digital gene expression showed that 24 out of the 37 transcripts presented in this study have significant changes in expression between eyestalk and brain. In some cases a trend of difference between males and females could be seen. Taken together, this study provides a comprehensive neuropeptidome of a commercially important crustacean species with novel peptides and protein hormones identified for the first time in decapods.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Fish Physiology and Genetics
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Crustaceans (excl. Rock Lobster and Prawns)
Author:Fitzgibbon, Q (Dr Quinn Fitzgibbon)
Author:Battaglene, S (Associate Professor Stephen Battaglene)
ID Code:91709
Year Published:2014
Web of Science® Times Cited:24
Deposited By:Sustainable Marine Research Collaboration
Deposited On:2014-05-27
Last Modified:2015-03-17
Downloads:192 View Download Statistics

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