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Hemolytic activity and fatty acids composition in the ichthyotoxic dinoflagellate Cochlodinium polykrikoides isolated from Bahía de La Paz, Gulf of California

journal contribution
posted on 2023-05-18, 00:59 authored by Dorantes Aranda, JJ, Garcia-de la Parra, LM, Alonso-Rodriguez, R, Morquecho, L
The hemolytic activity of the dinoflagellate Cochlodinium polykrikoides from Bahía de La Paz, Gulf of California was investigated as part of the ichthyotoxic mechanism of this microalga. Two different kinds of erythrocytes, fish and human, were tested for the hemolytic assay. Since fatty acids have been associated with hemolytic activity in C. polykrikoides, the composition of fatty acids of this dinoflagellate was also analyzed. The concentration of C. polykrikoides causing 50% hemolysis (HE50) was 4.88 and 5.27 × 106 cells L−1, for fish and human erythrocytes, respectively. According to the standard curve of saponin, an equivalence between the hemolytic activity of saponin and the dinoflagellate concentration was found with 1 μg saponin mL−1 equivalent to 1 × 106 cells L−1 of C. polykrikoides. The polyunsaturated fatty acids: hexadecaenoic (16:0), docosahexaenoic (22:6 n3) and octadecapentaenoic (18:5 n3) were found in an abundance of ∼62% of total fatty acids.

History

Publication title

Marine Pollution Bulletin

Volume

58

Pagination

1401-1405

ISSN

0025-326X

Department/School

Institute for Marine and Antarctic Studies

Publisher

Pergamon-Elsevier Science Ltd

Place of publication

The Boulevard, Langford Lane, Kidlington, Oxford, England, Ox5 1Gb

Rights statement

Copyright 2009 Elsevier Ltd.

Repository Status

  • Restricted

Socio-economic Objectives

Aquaculture fin fish (excl. tuna)

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