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Hemolytic activity and fatty acids composition in the ichthyotoxic dinoflagellate Cochlodinium polykrikoides isolated from Bahía de La Paz, Gulf of California
Citation
Dorantes Aranda, JJ and Garcia-de la Parra, LM and Alonso-Rodriguez, R and Morquecho, L, Hemolytic activity and fatty acids composition in the ichthyotoxic dinoflagellate Cochlodinium polykrikoides isolated from Bahia de La Paz, Gulf of California, Marine Pollution Bulletin, 58 pp. 1401-1405. ISSN 0025-326X (2009) [Refereed Article]
Copyright Statement
Copyright 2009 Elsevier Ltd.
DOI: doi:10.1016/j.marpolbul.2009.06.007
Abstract
The hemolytic activity of the dinoflagellate Cochlodinium polykrikoides from Bahía de La Paz, Gulf of California was investigated as part of the ichthyotoxic mechanism of this microalga. Two different kinds of erythrocytes, fish and human, were tested for the hemolytic assay. Since fatty acids have been associated with hemolytic activity in C. polykrikoides, the composition of fatty acids of this dinoflagellate was also analyzed. The concentration of C. polykrikoides causing 50% hemolysis (HE50) was 4.88 and 5.27 × 106 cells L−1, for fish and human erythrocytes, respectively. According to the standard curve of saponin, an equivalence between the hemolytic activity of saponin and the dinoflagellate concentration was found with 1 μg saponin mL−1 equivalent to 1 × 106 cells L−1 of C. polykrikoides. The polyunsaturated fatty acids: hexadecaenoic (16:0), docosahexaenoic (22:6 n3) and octadecapentaenoic (18:5 n3) were found in an abundance of ∼62% of total fatty acids.
Item Details
Item Type: | Refereed Article |
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Keywords: | hemolysis, Cochlodinium polykrikoides, dinoflagellate, harmful algal bloom |
Research Division: | Biological Sciences |
Research Group: | Ecology |
Research Field: | Marine and estuarine ecology (incl. marine ichthyology) |
Objective Division: | Animal Production and Animal Primary Products |
Objective Group: | Fisheries - aquaculture |
Objective Field: | Aquaculture fin fish (excl. tuna) |
UTAS Author: | Dorantes Aranda, JJ (Dr Juan Dorantes Aranda) |
ID Code: | 91567 |
Year Published: | 2009 |
Web of Science® Times Cited: | 26 |
Deposited By: | IMAS Research and Education Centre |
Deposited On: | 2014-05-22 |
Last Modified: | 2015-08-20 |
Downloads: | 0 |
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