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Hemolytic activity and fatty acids composition in the ichthyotoxic dinoflagellate Cochlodinium polykrikoides isolated from Bahía de La Paz, Gulf of California
journal contribution
posted on 2023-05-18, 00:59 authored by Dorantes Aranda, JJ, Garcia-de la Parra, LM, Alonso-Rodriguez, R, Morquecho, LThe hemolytic activity of the dinoflagellate Cochlodinium polykrikoides from Bahía de La Paz, Gulf of California was investigated as part of the ichthyotoxic mechanism of this microalga. Two different kinds of erythrocytes, fish and human, were tested for the hemolytic assay. Since fatty acids have been associated with hemolytic activity in C. polykrikoides, the composition of fatty acids of this dinoflagellate was also analyzed. The concentration of C. polykrikoides causing 50% hemolysis (HE50) was 4.88 and 5.27 × 106 cells L−1, for fish and human erythrocytes, respectively. According to the standard curve of saponin, an equivalence between the hemolytic activity of saponin and the dinoflagellate concentration was found with 1 μg saponin mL−1 equivalent to 1 × 106 cells L−1 of C. polykrikoides. The polyunsaturated fatty acids: hexadecaenoic (16:0), docosahexaenoic (22:6 n3) and octadecapentaenoic (18:5 n3) were found in an abundance of ∼62% of total fatty acids.
History
Publication title
Marine Pollution BulletinVolume
58Pagination
1401-1405ISSN
0025-326XDepartment/School
Institute for Marine and Antarctic StudiesPublisher
Pergamon-Elsevier Science LtdPlace of publication
The Boulevard, Langford Lane, Kidlington, Oxford, England, Ox5 1GbRights statement
Copyright 2009 Elsevier Ltd.Repository Status
- Restricted